BACKGROUND IgE-expressing (IgE+ ) plasma cells (PCs) provide a continuous source of allergen-specific IgE that is central to allergic responses. The extreme sparsity of IgE+ cells in vivo has confined their study almost entirely to mouse models. OBJECTIVE To characterize the development pathway of human IgE+ PCs and to determine the ontogeny of human IgE+ PCs. METHODS To generate human IgE+...

Induction of IgE class-restricted tolerance was studied in high IgE-responder (BALB/c X SJL)F1 mice, of which the parental BALB/c and SJL mice are high and low IgE-responder mice, respectively. 2,4-Dinitrophenyl (DNP)-specific monoclonal IgE was administered to (BALB/c X SJL)F1 mice neonatally in two forms: soluble IgE at 250 micrograms per injection, or 10-100 ng of IgE coupled to 25-50 X 10(6...

serum ige assay is a mainstay step in the allergy work up. allergenic extracts and molecular components are available at present. this real life study compared the serum specific ige levels against allergenic extracts with allergenic molecules in patients allergic to parietaria, betulaceae , and mites. this retrospective real life study included 489 subjects with respiratory allergy. inclusion ...

determining the normal range of immunoglobulin e (ige) in any population is important for diagnosis of allergic diseases. the aim of this study was to determine normal values of ige in children living in tehran. two-handed and fifty six healthy children aged one month to 14 years were selected. these children had neither parasitic infections nor atopic disease. they were divided into six groups...

background: nasal polyps, a common clinical problem, are characterized by eosinophilic and mast cell inflammation.  the role of allergy and ige in pathogenesis of nasal polyps is still unclear.  ige receptors are important components of the immunological pathway in allergic and inflammatory diseases.   objective: to determine if the low affinity ige receptor (cd23) is presented on nasal polyp t...

IgE antibodies with high affinity for their antigens can be stably cross-linked at low concentrations by trace amounts of antigen, whereas IgE antibodies with low affinity bind their antigens weakly. In this study, we find that there are two distinct pathways to generate high and low affinity IgE. High affinity IgE is generated through sequential class switching (μ→γ→ε) in which an intermediary...

The discovery of IgE represented a major breakthrough in allergy and asthma research, whereas the clinical interest given to IgE in asthma has been blurred until the arrival of anti-IgE biotherapy. Novel facets of the complex link between IgE and asthma have been highlighted by the effect of this treatment and by basic research. In parallel, asthma phenotyping recently evolved to the concept of...

IgE-mediated cell signaling, induced by cross-linking of high affinity receptor for IgE (FcεRI) in the presence of antigen (Ag), is a well known mechanism described for mast cell activation in allergy and hypersensitivity reactions, which induces a spectrum of cellular responses such as secretion and up-regulation of cell surface FcεRI. Although for several years IgE binding to FcεRI was consid...

Measurement of allergen-specific immunoglobulin E (IgE) is a common practice in the investigation of allergy. It has not been possible to measure porcine IgE due to unavailability of anti-porcine IgE. This study was undertaken to purify and characterize porcine IgE from sera of allergic pigs, identify heterologous anti-IgE reactive with pig IgE and to use purified heavy (H) chain of porcine IgE...