BACKGROUND Oxidative stress plays a role in the development of chronic peripheral arterial disease (PAD) because under these conditions redox regulation is impaired, inducing the S-glutathionylation of proteins. A method of estimating the levels of S-glutathionylated proteins has been developed using biotinylated glutathione S-transferase, which allows the study of their crucial role in the oxi...

The in vivo potency of antisense oligonucleotides (ASO) has been significantly increased by reducing their length to 8-15 nucleotides and by the incorporation of high affinity RNA binders such as 2', 4'-bridged nucleic acids (also known as locked nucleic acid or LNA, and 2',4'-constrained ethyl [cET]). We now report the development of a novel ASO design in which such short ASO monomers to one o...

Diets with high fat content induce steatosis, insulin resistance, and type 2 diabetes. The lipid droplet protein adipose differentiation-related protein (ADRP) mediates hepatic steatosis, but whether this affects insulin action in the liver or peripheral organs in diet-induced obesity is uncertain. We fed C57BL/6J mice a high-fat diet and simultaneously treated them with an antisense oligonucle...

Embolization of the occlusion device after percutaneous closure of atrial septal defect (ASD) is a potential disastrous complication. The usual site of embolization is the right side of the heart including pulmonary artery, but the device embolization to the extracardiac aorta is extremely rare. Here, we report a successful percutaneous retrieval case of the embolized Amplatzer Septal Occluder ...

The title compound, sodium dioxidomolybdenum(VI) arsenate(V), β-NaMoO(2)AsO(4), was prepared by solid-state reaction at 953 K. In the crystal structure, the AsO(4) tetra-hedra and MoO(6) octa-hedra (both with m symmetry) share corner atoms to form a three-dimensional framework that delimits cavities parallel to [010] where disordered six-coordinated sodium cations (half-occupation) are located....

نقش صلاحیت فردی و رضایت شغلی ممیزان مالیاتی در ارتقاء سطح قدرت "مالیات‌ستانی" توسط سازمان امور کشور: بررسی محدودیت (فشار) زمان

PURPOSE The tumor microenvironment is complex and heterogeneous, populated by tortuous irregular vasculature, hypoxic cells, and necrotic regions. These factors can all contribute to the biodistribution difficulties encountered by most cancer therapeutic agents. Antisense oligodeoxynucleotides (ASO) are a class of therapeutics where limited information is available about their distribution with...

Machine learning (ML) has been increasingly used to aid aerodynamic shape optimization (ASO), thanks the availability of data and continued developments in deep learning. We review applications ML ASO date provide a perspective on state-of-the-art future directions. first introduce conventional current challenges. Next, we fundamentals detail algorithms that have successful ASO. Then, addressin...

The anti-invasive activity of antisense oligonucleotides (ASO) specific to the K-ras gene in hamster pancreatic cancer was investigated. HaP-T1, a cell culture derived from BHP-induced hamster pancreatic cancer, was used. After liposome-mediated transfection with mutation-matched and mutation-mismatched ASO in different concentrations, cell proliferation was studied by MTT and MTT-agarose metho...

The streptozyme test was compared with the antistreptolysin O (ASO), antideoxyribonuclease B (ADN-B), and antihyaluronidase (AH) tests. One hundred and twenty-seven human serum specimens were tested by each of the four tests. The streptozyme test detected more specimens with elevated titers than any single test; however, it was not as effective as the combination of the ASO and ADN-B tests. The...