The competitive adsorption of proteins and surfactants has applications to chromatographic systems and biological materials. Adsorption for systems of bovine serum albumin (BSA) and sodium myristate (SM) was investigated with in-situ ATR-IR spectroscopy and ex-situ ellipsometry. The results were used to determine quantitatively the surface densities of the adsorbates at the surface. For a mixtu...

Studies were undertaken to determine whether BSA, once immobilized on activated nylon microspheres, would be capable of specifically removing circulating BSA antibody in vitro and in vivo in an extracorporeal circulation system in dogs. Nylon microspheres were prepared and, after gentle hydrolysis and glutaraldehyde treatment, demonstrated a retention of up to 34.5 mg of BSA. In vitro studies s...

In this paper, the interaction of lomefloxacin (LMF) and/or cefazolin (CFZ) with bovine serum albumin (BSA) was studied by fluorescence quenching in combination with UV-vis spectroscopic method under simulative physiological conditions. The fluorescence quenching constants, binding distance, and binding constants for BSA–LMX and/or CFZ systems were determined. The fluorescence quenching of BSA ...

using uv-vis spectrophotometric method the interaction of water soluble phthalocyanine, cobalt(ιι) 4,4′,4′′,4′′′- tetrasulfophthalocyanine(cotspc), with bovine serum albumin (bsa) to determine the formation constant and related thermodynamic functions. the measurements were considered in 1mm sodium phosphate buffer, ph 7.0 and at 5 different temperatures 20, 25, 30, 35 and 40ºc. the results sho...

We have investigated the nongenomic as well as the genomic effects of glucocorticoids on phosphate (Pi) uptake in primary rabbit renal proximal tubule cells (PTCs) and have defined the involved signaling pathways. In the present study, cortisol-BSA (cortisol-BSA) (>10(-9) M, 30 min) was found to inhibit Pi uptake in a time- and concentration-dependent manner. However, progesterone-BSA (P(4)-BSA...

In vitro culture for bovine embryos is largely not optimal. Our study was to determine the components necessary for early embryo development. In experiment 1, IVF embryos were cultured for two days in CR1aa medium containing sodium citrate and BSA from two sources (Sigma vs. ICPbio), subsequently for additional five days with cumulus monolayer in 10% FBS CR1aa. We found that supplementation wit...

BACKGROUND Physiological functions are often assessed by standardizing for body surface area (BSA) to avoid excessive variation in calculations in pediatric practice. AIM To explore the suitability of existing formulae for estimating the BSA of Nigerian children. SUBJECTS AND METHODS This cross-sectional study involved healthy children in a Local Government Area, Oyo State, Nigeria. The BSA...

Dietary flavonoids show beneficial effects in the prevention of chronic diseases. However, flavonoid bioavailability is poor, probably due to their interaction with serum albumins. In the current work, the binding interactions of eight related flavonoids, sharing a similar core structure, with bovine serum albumin (BSA) were investigated by fluorescence spectroscopy. The binding affinities of t...

Abstract: The aim of the present study was to produce a polyclonal antibody against bovine serum albumin (BSA) conjugated with artemisinin. To gain an immunogenic character of artemisinin, a carboxyl group was added to it using mixed anhydride method. Then, the reactive compound of artemisinin was conjugated with BSA. The BSA+artemisinin were injected to white female New Zealand rabbits for two...