We developed an efficient and fast method based on a protein microarray integrated with a microfluidic chip for the process of SELEX (systematic evolution of ligands by exponential enrichment). Lactoferrin from bovine milk was used as a target protein, while bovine serum albumin (BSA), α-lactalbumin, β-lactoglobulin and casein were used as negative proteins. They were separately dotted and immo...

Objective: Primary Malignant Melanoma (PMM) arising from the digestive, respiratory or genitourinary tract is extremely rare. Rectal PMM accounts for less than 1% of all melanomas; it is often advanced at initial presentation with a poor prognosis with a 5-year survival below 20%. Treatment of this particular disease is still debated with weak evidence that aggressive surgery may lead to a bett...

Penicillium marneffei is an emerging opportunistic dimorphic fungal pathogen that is endemic in Southeast Asia. A typing method based on the analysis of size polymorphisms in microsatellite loci was investigated. Three loci available from the GenBank database were identified to harbor microsatellites. PCR primers flanking the microsatellite repeats were designed with one primer in the set fluor...

Central Pacific (CP) and Eastern Pacific (EP) type ENSO events are inves-2 tigated using linear inverse modeling (LIM). Optimal initial conditions and growth 3 rates for CP or EP ENSO events are identified explicitly using a CP or EP ENSO 4 norm. The dominant difference in initial conditions that lead to CP and EP ENSO 5 events is the role of the second empirical orthogonal function of tropical...

Modern organizations are challenged to enact a digital transformation and improve their competitiveness while contributing the ninth Sustainable Development Goal (SGD), “Build resilient infrastructure, promote sustainable industrialization foster innovation”. The discovery of hidden process data’s knowledge assets may help digitalize processes. Working on valuable asset process, we found major ...

Abstract Market making (MM) is an important means of providing liquidity to the stock markets. Recent research suggests that reinforcement learning (RL) can improve MM significantly in terms returns. In latest work on RL-based MM, reward a function equity returns, calculated based its current price, and inventory agent. As result, agent’s return maximised provided. If price movement known this ...

The diagnosis of primary mitochondrial myopathy is often delayed by years due to non-specific clinical symptoms as well variable testing disorders. aim this review summarize and discuss the collective findings novel insights regarding diagnosing, testing, presentation (PMM). PMM results from a disruption oxidative phosphorylation (OXPHOS) chain in mitochondria mutations DNA (mtDNA) or nuclear (...

Extratropical atmospheric variability can impact tropical climate in the Pacific sector via the Pacific Meridional Modes (PMMs). The South PMM (SPMM) has a larger equatorial signature than the North PMM (NPMM) for the same amount of extratropical variability. Here we explain this interhemispheric asymmetry using an atmospheric general circulationmodel coupled to a slab ocean model. By imposing ...

In this paper, by using the perturbed matrix method (PMM) in combination with basic statistical mechanical relations both based on nanosecond time-scale molecular dynamics (MD) simulations, we quantitatively address the thermodynamics of compound 0 (Cpd 0) formation in horseradish peroxidase (HRP) enzyme. Our results, in the same trend of low-temperature experimental data, obtained in cryoenzym...

Even when employees use project management methodologies (PMMs), the question regarding the nature of their use remains, i.e. are they using PMMs in a dedicated manner? While empirical research states that the usefulness of a methodology is the single most important determinant of its acceptance and use by actual users, studies have not examined which methodology use outcomes are more important...