Pig muscle phosphoglucose isomerase modified with pyridoxal 5'-phosphate under conditions that cause at least 90% inactivation of its catalytic activity was found to incorporate about 1.5 eq of pyridoxal 5'-phosphate per subunit. After digestion with thermolysin, two pyridoxal 5'-phosphate-containing peptides were isolated and their amino acid sequences were determined to be Leu-Gly-pyridoxyl-L...

The sequences of the bla(TEM) genes encoding TEM-92 in Proteus mirabilis and Providencia stuartii isolates were determined and were found to be identical. Except for positions 218 (Lys-6) and 512 (Lys-104), the nucleotide sequence of bla(TEM-92) was identical to that of bla(TEM-20), including the sequence of the promoter region harboring a 135-bp deletion combined with a G-162-->T substitution....

The current wound-healing collagen mimetic peptides (CMPs) have limitations such as poor membrane permeability and protease susceptibility. Herein, the solid-phase peptide synthesis of CMPs containing integrin binding motif GFOGER is reported. sequences also consist lipophilic moieties (adamantane palmitic acid) for improved different collagen-inducing tripeptides, namely, Thr-Thr-Lys (TTK), Gl...

The SARS-CoV-2 main protease (Mpro) is essential for the life cycle of COVID-19 virus. It cleaves two polyproteins at 11 positions to generate mature proteins virion formation. cleavage site on these known be Leu-Gln↓(Ser/Ala/Gly). A range hexapeptides that follow sequence recognition and was constructed using RDKit libraries complexed with crystal structure Mpro (PDB ID 6XHM) through extensive...

Sequence analysis was performed of an ovine hypothalamic 41-residue polypeptide that had been postulated to be a putative corticotropin-releasing factor (CRF) because of its high intrinsic corticotropin releasing activity. The NH2-terminal 39 residues of CRF were determined by Edman degradation of 0.6-3.5 nmol of peptide in a Wittmann-Liebold modified Beckman 890C spinning cup sequencer with re...

Tobacco smoking and occupational exposures are the main known risk factors for bladder cancer, causing direct and indirect damage to DNA. Repair of DNA damage is under genetic control, and DNA repair genes may play a key role in maintaining genome integrity and preventing cancer development. Polymorphisms in DNA repair genes resulting in variation of DNA repair efficiency may therefore be assoc...

This study deals with the interaction of polypeptide hormones with their receptors. Specifically it involves the binding of synthetic ACTH analogs and fragments to a particulate fraction from beef adrenal cortical tissue. This fraction was found to bind synthetic [(14)C-Phe] [Gln(5)]beta-corticotropin(1-20) amide but failed to bind significant amounts of [(14)C-Phe] [Gln(5)]beta-corticotropin(1...

A Glu-89-->Gly alteration in the human immunodeficiency virus type 1 reverse transcriptase (RT) was previously shown to result in resistance to several dideoxynucleoside analogs and to phosphonoformic acid (PFA; foscarnet). This residue was altered to Ala, Val, Ser, Thr, Gln, Asp, Asn, or Lys, and the ddGTP and PFA sensitivities of the mutant RTs were measured. Replacements with Ala, Gly, Val, ...