Single nucleotide polymorphisms (SNPs) are the most commonly used polymorphic markers in genetics studies. Among the different platforms for SNP genotyping, Luminex is one of the less exploited mainly due to the lack of a robust (semi-automated and replicable) freely available genotype calling software. Here we describe a clustering algorithm that provides automated SNP calls for Luminex genoty...

Antilymphocyte globulin (ALG) was given every other day for 5 doses with platelet transfusions immediately following ALG administration in 6 patients with aplastic anaemia. Four patients responded and 3 durable remissions were achieved. One patient relapsed and further treatment with anti-thymocyte globulin and cyclosporin also failed. One patient died of Flavobacterium septicaemia 6 days after...

Blum, Cucker, Shub and Smale have shown that the \P = NP ?" problem has the same answer in all algebraically closed elds of characteristic 0. We generalize this result to the polynomial hierarchy: if it collapses over an algebraically closed eld of characteristic 0, then it must collapse at the same level over all algebraically closed elds of characteristic 0. The main ingredient of their proof...

  During last two decades, polysaccharides such as alginate (Alg) alone and in combination with other biopolymers are widely used in vaccine and drug delivery systems. The aim of the present work was to investigate the potential utility of microparticles made of alginate (Alg) as new vehicles for improving nasal vaccine delivery. For this purpose, diphtheria toxoid (DT) was chosen as a model an...

BACKGROUND Basiliximab is a chimeric human/mouse monoclonal antibody directed against the alpha chain of the IL-2 receptor, CD25, which has been reported as successfully reducing rejection in adult renal transplant recipients. Reported clinical experience of basiliximab in paediatric renal transplantation is limited. METHODS Using two intravenous doses on day 0 (pre-operatively) and day 4 wit...

M icroarray tech niq ues h av e motiv ated th e d ev elop of d iff erent meth od s to ex tract useful information from a b iolog ical point of v iew . B iclustering alg orith ms ob tain a set of g enes w ith th e same b eh av iour ov er a g roup of ex perimental cond itions from g ene ex pression d ata. In ord er to ev aluate th e q uality of a b icluster, it is useful to id entify specifi c te...

RNAi is a gene-silencing phenomenon triggered by double-stranded (ds) RNA and involves the generation of 21 to 26 nt RNA segments that guide mRNA destruction. In Caenorhabditis elegans, lin-4 and let-7 encode small temporal RNAs (stRNAs) of 22 nt that regulate stage-specific development. Here we show that inactivation of genes related to RNAi pathway genes, a homolog of Drosophila Dicer (dcr-1)...

Ekoturizm do?al-kültürel de?erleri koruyan, kal?c? zararlar vermeden yerel halk?n ekonomik gelir elde edebilecek ?ekilde kullanmas?na imkân sa?layan ve ayn? zamanda sosyo-kültürel ili?kilere olumlu katk?s? olan bir turizm türüdür. ülke bölge aç?s?ndan büyük öneme sahiptir. Ayr?ca hareketlili?ini hem 12 aya de geneline yayabilecek potansiyele Ekoturizmin bölgede geli?ebilmesi o ya?ayan alg?s?na ...

Polyethyleneimine (PEI)-alginate (Alg) nanoparticle (NP) is a safe and effective vector for delivery of siRNA or DNA. Recent studies suggest that autophagy is related to cytotoxicity of PEI NPs. However, contribution of autophagy to degradation of PEI-Alg NPs remains unknown. CD34+VEGFR-3+ endothelial progenitor cells isolated from rat bone marrow were treated with 25 kDa branched PEI modified ...