1 Abstract Abstract Neutrophil granulocytes are one of the first lines of defense of the mammalian innate immune system against invading microorganisms. In response to inflammatory stimuli, neutrophils migrate from the blood stream to infected tissues where they bind, engulf and inactivate microorganisms efficiently. Human neutrophil elastase (NE), a neutrophil granule component, is a key host ...

Abstract Study Objective: Here, we utilize a nanobody yeast display library to identify nanobodies that target virulence factors in Shigellaspp. and Salmonella enterica typhimuriumand determine if these (Nbs) can inhibit host cell invasion vitro. Brief Statement of Methods: Using synthetic containing camelid Nbs with randomized CDR loops, selected for binders select Enterobacteriaceae proteins,...