Recently we have isolated the genomic clones of rat cytochrome c oxidase subunit IV and determined the structural organization of the gene (1). Here we report the complete nucleotide sequence of the gene encoding rat cytochrome c oxidase subunit IV for the record. The rat cytochrome c oxidase subunit IV gene is composed of 6,106 nucleotides and is organized in five exons (exon 1; 44 bp, exon 2;...

the effect of annona squamosa, lawsonia inermis and their combination with other herbal molluscicides were studied on different enzyme activity in the nervous tissue of lymnaea acuminata. twenty-four hour in vivo exposure to 40% and 80% of 24 h lc50 of plant derived molluscicides and their combination with other molluscicides such as cedrus deodara, azadirachta indica oil, allium sativum, polia...

The aim of the study was to determine the activity of cytochrome c oxidase in mitochondrial fractions of cataractogenic epithelial cells of lenses of 60 patients (34 females and 26 males). According to clinical criteria patients were divided into three groups: group 1--patients with senile cataract, group 2--active smokers with senile cataract, and group 3--diabetic patients with cataract as di...

Liver microsomes contain a mixed function oxidase system capable of metabolizing a broad range of biologically active substances, including drugs, steroids, and environmental chemicals such as polycyclic hydrocarbons. Certain hemoproteins, collectively referred to as cytochrome P-450, function as the terminal oxidase in this system. The fact that this hepatic mixed function oxidase system is se...

We investigated the effects of dexamethasone or indomethacin on the NADPH oxidase activity, cytochrome b558 content, and expression of genes encoding the components gp91-phox and p47-phox of the NADPH oxidase system in the human monocytic THP-1 cell line, differentiated with IFN-gamma and TNF-alpha, alone or in combination, for up to 7 days. IFN-gamma and TNF-alpha, alone or in combination, cau...

Cytochrome c oxidase isolated from beef heart mitochondria was reconstituted in bilayer membranes of the anionic lipid dimyristoylphosphatidylglycerol (DMPG) with varying enzyme/DMPG ratio. Lipid-protein interactions in the reconstituted membrane complexes were studied in the presence and absence of saturating amounts of bound cytochrome c, by both chemical binding assays and spin-label ESR spe...

The cytochrome c oxidase (COX) is the terminal enzyme of the respiratory chain. The complex accepts electrons from cytochrome c and passes them onto molecular oxygen. This process contributes to energy capture in the form of a membrane potential across the inner membrane. The enzyme complex assembles in a stepwise process from the three mitochondria-encoded core subunits Cox1, Cox2 and Cox3, wh...

Purified and reconstituted cytpchrome ~oxidase and mitochondria were crosslinked with biimidates in the presence and absence of cytochrome c. These experiments indicate that oxidase subunit interactions are required for activity and that cytochrome~ mobility may be required for electron transport activity. Biimidate treatment of purified and reconstituted oxidase crosslinks all of the oxidase p...

The biogenesis of multimeric protein complexes of the inner mitochondrial membrane in yeast requires a number of nuclear-coded ancillary proteins. One of these, Pet100p, is required for cytochrome c oxidase. Previous studies have shown that Pet100p is not required for the synthesis, processing, or targeting of cytochrome c oxidase subunits to the mitochondrion nor for heme A biosynthesis. Here,...