The first enzyme of the pathway for uridine diphosphate N-acetyl-D-glucosamine (UDPAG) biosynthesis i.e. L-glutamine: D-fructose 6-P amidotransferase (E.C. 2.6.1.16) was purified 52-fold from human placenta using methanol fractionation and column chromatography on DEAE-Sephadex A-50. The enzyme showed optimal activity in a broad range of pH from 5.8 to 7.8 in both phosphate and cacodylate ...

Abstract. For any odd squarefree integer r, we get complete description of the Gr = Gal(Q(μr)/Q) group cohomology of the universal ordinary distribution Ur in this paper. Moreover, if M is a fixed integer which divides l−1 for all prime factors l of r, we study the cohomology group H(Gr, Ur/MUr). In particular, we explain the mysterious construction of the elements κr′ for r |r in Rubin[10], wh...

1. Pyruvate kinase purified from the hepatopancrease of Carcinus maenas exhibited sigmoidal saturation kinetics with respect to the substrate phosphoenolpyruvate in the absence of the allosteric activator fructose 1,6-bisphosphate, but normal hyperbolic saturation was seen in the presence of this activator. The activation appears to be the result of a decrease in the s0.5 (phosphoenolpyruvate) ...

Reductions of N -wave type equations related to simple Lie algebras and the hierarchy of their Hamiltonian structures are studied. The reduction group GR is realized as a subgroup of the Weyl group of the corresponding algebra. Some of the reduced equations are of physical interest. 1. Preliminary. The analysis and the classification of all reductions for the nonlinear evolution equations solva...

Although both alcohol and fructose are particularly steatogenic, their long-term effect in the development of a metabolic syndrome has not been studied in vivo. Consumption of fructose generally leads to obesity, whereas ethanol can induce liver damage in the absence of overweight. Here, Sprague-Dawley rats were fed ad libitum for 28 days on five diets: chow (control), liquid Lieber-DeCarli (LD...

Fructose in excessive amounts exerts negative effects on insulin sensitivity, blood pressure, and liver metabolism. These adverse outcomes were attributed to its disturbances of key metabolic pathways in the liver. Recently, possible consequences of high fructose levels directly on adipocytes in vivo have been considered. We have cultured adipocytes in growth media containing 1 g/L fructose add...

BACKGROUND This study was performed to investigate expression and distribution of glucocorticoid receptor (GR) in the rat adrenal cortex, acute effect of ethanol on its expression and possible role of endogenous nitric oxide (NO) in this phenomenon. METHODS Adult female Wistar rats showing diestrus day 1 were treated with: a) ethanol (2 or 4 g/kg body weight (b.w.), ip), b) N(ω)-nitro-L-argin...

The demonstration (l-3) of a distinctive pathway of fructose metabolism in liver via the aldol cleavage of fructose l-phosphate emphasized the possibilty in this tissue of an enriched catalytic system for the facilitation of this reaction since the classical fructose-l ,gdiphosphate aldolase isolated from muscle tissue exhibits relatively little activity toward fructose l-phosphate. The early e...

Coproduction of fructose and ethanol from dates extract by a glucose-selective S. cerevisiae ATCC 36859 strain has been studied. Various initial sugar concentrations (i.e., 131.4, 315.3, 408.2, and 500.0 g/l) have been tested. The fermentation experiments were performed in a water shaker bath at 30°C and 120 rpm. The results showed that highest yields of fructose (95.0%) and ethanol (72.8%) wer...