TP2 was purified from rat testes employing a gentle method involving differential salt extraction of the sonication-resistant spermatid nuclei. The nucleic acid binding properties of TP2 were studied by fluorescence quenching, thermal denaturation, circular dichroism techniques and compared with those of TP1 (Singh, J., and Rao, M. R. S. (1987) J. Biol. Chem. 262, 734-740). The tyrosine fluores...

Area postrema lesions (APX) in adult male rats produced a robust spontaneous intake of 0.5 M NaCl, as reported previously. The largest NaCl intakes (up to 108 ml/day) were observed when there was little incidental damage in the medial subnucleus of the nucleus of the solitary tract adjacent to the caudal and middle portions of the area postrema. Rats with discrete APX also drank substantial amo...

Neural activity in intact chorda tympani nerve of rats was studied with an electronic summator. Neural activity increased when amino acid solutions 0.01 M or above passed over the tongue. Response magnitude, at concentrations close to solubility limits for the amino acids tested, was: DL-methionine < DL-tryptophan < DL-valine < DL-alanine < glycine < 0.1 M NaCl. Maximum response magnitudes to 1...

In experiments with the K strain of Staphylococcus aureus and the K race of bacteriophage suspended in tryptose phosphate broth and maintained at 42 degrees C. it was found that the presence of 1 M NaCl produced certain drastic changes in the relationship between the host cells and the infecting virus: 1. Staphylococci grown at 42 degrees C. in plain broth or in NaCl-broth are "activated," i.e....

Rats drank rapidly when 0.3 M NaCl was the only drinking fluid available after overnight water deprivation, consuming approximately 200 ml/24 h. Although such large intakes of this hypertonic solution initially elevated plasma osmolality, excretion of comparable volumes of urine more concentrated than 300 meq Na(+)/l ultimately appears to restore plasma osmolality to normal levels. Rats drank a...

The main purpose of the two consecutive experimental studies presented here was to compare effect salinity on nutrients in leaves halophytic plant species Portulaca oleracea L. and soil. first experiment conducted study growth, biomass accumulation, yield, root layer development, salt dynamics changes mineral substances plants In second experiment, P. seeds were sown directly into salinized soi...

Factor VIII activation by thrombin is the result of a proteolytic cleavage of the procoagulant component. These studies examine the effect of human antibody on this activation step in a solid phase immunoadsorbent assay system. Radiolabeled factor VIII antibody: factor VIII protein immune complexes were bound to agarose beads by mouse monoclonal antifactor VIII R:Ag antibody. The incubation of ...

The objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular L-asparaginase. A total number of 120 halophilic bacteria were isolated from 17 different saline habitats of Iran including salt lakes, wetlands, brine springs and deserts. Among these, 68 were able to grow in the presence of 1.5 M NaCl and 52 demonstrated the ability to gro...

the objective of this study was to isolate halophilic bacteria with the ability to produce intracellular or extracellular l-asparaginase. a total number of 120 halophilic bacteria were isolated from 17 different saline habitats of iran including salt lakes, wetlands, brine springs and deserts. among these, 68 were able to grow in the presence of 1.5 m nacl and 52 demonstrated the ability to gro...

in this study, salinity tolerance of 51 kabuli chickpea genotypes was investigated at vegetative growth phase using 4.5 and 6.5 ds/m salt solution (by adding nacl to 1/2 hoagland solution) and 1/2 hoagland solution (ec= 1.7 ds/m) as control treatment. a split plot design was used in this experiment where the main plot was salinity treatments and the sub plot was genotypes. treatments continued ...