نتایج جستجو برای: 16s rrna hp1

تعداد نتایج: 36763  

Journal: :Antimicrobial Agents and Chemotherapy 2008

Journal: :Journal of bacteriology 1996
M La Farina S Stira R Mancuso C Grisanti

Streptomyces venezuelae ATCC 10595 harbors seven rRNA gene clusters which can be distinguished by BglII digestion. The three rRNA genes present in each set are closely linked with the general structure 16S-23S-5S. We cloned rrnA and sequenced the 16S-23S spacer region and the region downstream of the 5S rRNA gene. No tRNA gene was found in these regions.

Journal: :Applied and environmental microbiology 2007
Rebecca J Case Yan Boucher Ingela Dahllöf Carola Holmström W Ford Doolittle Staffan Kjelleberg

Several characteristics of the 16S rRNA gene, such as its essential function, ubiquity, and evolutionary properties, have allowed it to become the most commonly used molecular marker in microbial ecology. However, one fact that has been overlooked is that multiple copies of this gene are often present in a given bacterium. These intragenomic copies can differ in sequence, leading to identificat...

2013
Tatsuya Tada Tohru Miyoshi-Akiyama Yasuyuki Kato Norio Ohmagari Nozomi Takeshita Nguyen Viet Hung Doan Mai Phuong Truong Anh Thu Nguyen Gia Binh Nguyen Quoc Anh Tran Thi Thanh Nga Pham Hong Truong Phan Thi Xuan Le Thi Anh Thu Nguyen Truong Son Teruo Kirikae

BACKGROUND 16S rRNA methylase-producing Gram-negative bacteria are highly resistant to all clinically important aminoglycosides. We analyzed clinical strains of 16S rRNA methylase-producing Acinetobactor baumannii and Pseudomonas aeruginosa obtained from clinical isolates in medical settings in Vietnam. METHODS From 2008 to 2011, 101 clinical strains of A. baumannii and 15 of P. aeruginosa we...

Journal: :Applied and environmental microbiology 2013
Dong-Lei Sun Xuan Jiang Qinglong L Wu Ning-Yi Zhou

Ever since Carl Woese introduced the use of 16S rRNA genes for determining the phylogenetic relationships of prokaryotes, this method has been regarded as the "gold standard" in both microbial phylogeny and ecology studies. However, intragenomic heterogeneity within 16S rRNA genes has been reported in many investigations and is believed to bias the estimation of prokaryotic diversity. In the cu...

Journal: :Journal of bacteriology 1997
Y Wang Z Zhang N Ramanan

Here we present the first description of the presence of two distinct types of 16S rRNA genes in the genome of a (eu)bacterium, Thermobispora bispora. We cloned and determined the nucleotide sequences of all four rRNA operons of T. bispora. Sequence comparisons revealed that the genome of T. bispora contains two distinct types of 16S rRNA genes, each type consisting of two identical or nearly i...

Journal: :Journal of clinical microbiology 1999
A Guimaraes-Peres F Portaels P de Rijk K Fissette S R Pattyn J van Vooren P Fonteyne

Two nested PCRs for the detection of Mycobacterium ulcerans were compared by using a collection of 65 clinical specimens. The first method amplifies the gene coding for 16S rRNA, and the second method amplifies a repetitive DNA sequence. The sensitivities of bacterioscopy, culture, 16S rRNA gene PCR, and repetitive-sequence PCR were 29, 34, 80, and 85%, respectively. Compared to the 16S rRNA ge...

Journal: :Nucleic acids research 2002
Thean Hock Tang Timofey S Rozhdestvensky Béatrice Clouet d'Orval Marie-Line Bortolin Harald Huber Bruno Charpentier Christiane Branlant Jean-Pierre Bachellerie Jürgen Brosius Alexander Hüttenhofer

The bulge-helix-bulge (BHB) motif recognised by the archaeal splicing endonuclease is also found in the long processing stems of archaeal rRNA precursors in which it is cleaved to generate pre-16S and pre-23S rRNAs. We show that in two species, Archaeoglobus fulgidus and Sulfolobus solfataricus, representatives from the two major archaeal kingdoms Euryarchaeota and Crenarchaeota, respectively, ...

Journal: :Emerging Infectious Diseases 2009
Katariina Vuorenmaa Iskandar Ben Salah Vincent Barlogis Hervé Chambost Michel Drancourt

Direct examination † Culture Molecular identification Ring of epithelial cells Giant cells Caseous necrosis Altered leukocytes Reference M. tuberculosis ± + IS6110 + + + – (6) M. avium 183 NR NR NR + NR + + NR NR (7) M. avium subsp. hominissuis 34 NR NR NR + IS1245 NR NR NR NR (5) M. haemophilum 39 NR NR + + 16S–23S rDNA + + + – (8) M. lentiflavum 3 SE Cured + + 16S rRNA + + NR + (9) M. bohemic...

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