Rat brain capillary endothelial (B10) cells express an unidentified nucleotide receptor linked to adenylyl cyclase inhibition. We show that this receptor in B10 cells is identical in sequence to the P2Y(12) ADP receptor ("P2Y(T)") of platelets. When expressed heterologously, 2-methylthio-ADP (2-MeSADP; EC(50), 2 nm), ADP, and adenosine 5'-O-(2-thio)diphosphate were agonists of cAMP decrease, an...

Two new TTF-based ligands involving terpyridine moiety (L1=4-(4′-Methyl-phenyl-2,2 : 6’,2’’-terpyridine −4-ylmethylthio)-5-(2-cyanoethylthio)-4′,5′-bis(methylthio)tetrathiafulvene and L2=4-(4′-Methyl-phenyl-2,2 −4-ylmethylthio)-5-(2-cyanoethylthio)-4′,5′-bis(propylthio)tetrathiafulvene) have been synthesized seven related mononuclear complexes were isolated as single crystals. The 2 1 (ligand m...

The kinetics and mechanism for degradation of omethoate (OMT) by catalytic ozonation with Fe(III)-loaded activated carbon (Fe@AC) were investigated in this study with focus on identification of degradation byproducts. The rate constants of OMT reacting with ozone and hydroxyl radical ((·)OH) were determined to be 0.04 and 5.3×10(8) M(-1) s(-1) at pH 7.5 and 20 °C, respectively. OMT was predomin...

The effects of the purine nucleotides, adenosine 5'-triphosphate (ATP) and their analogs 2-methylthio ATP and beta, gamma-methylene ATP, as well as those of the pyrimidine nucleotide, uridine 5'-triphosphate (UTP), on acid production in isolated rabbit gastric parietal cells prepared by enzymatic dispersion and enriched by counterflow elutriation were studied. The (14C)-aminopyrine (AP) accumul...

The present study employed standard peroxidase immunohistochemistry to map the distribution of P2Y(1) receptors in the rat brainstem and nodose ganglia and characterised the binding profile of [alpha(33)P]dATP. Binding of [alpha(33)P]dATP was fully displaceable by adenosine 5'-triphosphate (ATP), and was found on both human and rat nodose ganglia, and throughout the rat brainstem, including the...

The lethal effects of endotoxin, a bacterial product shed into the blood during bacteremia, are thought to be due to macrophage release of mediators such as tumor necrosis factor alpha and interleukin 1. Although much is known about the pathophysiology of endotoxemia, relatively little is known about the cellular signaling mechanisms that are involved. The data in this study suggest that extrac...