dna typing is a new method with important applications in forensic medicine. in the present study, we evaluated application of dna typing in iran. loci hum lpl, hum tpox, hum f13, hum vw 31a, hum th01 and hum fes/fps of dna short tandem repeats were studied. to determine sensitivity of the test, 85 mother-child couples (1020 chromosomes) that were referred to dna section of legal medicine organ...

Deletions of loci on chromosome 11p have been found frequently in several malignant tumors including gliomas, suggesting the presence of tumor suppressor genes. We analyzed 38 gliomas [26 malignant gliomas (grades III and IV) and 12 less malignant gliomas (grade I and II)] for loss of heterozygosity using microsatellite sequences on 11p as polymorphic markers. Loss of heterozygosity was found i...

Berari goats are medium sized animals reared mainly for meat purpose. They are predominantly brown and blackish brown in colour with glossy hair and found in Vidarbha region of Maharashtra state. Berari is not a recognized breed but a well established local population of goat which is yet to be fully explored for its phenotypic and genetic aspects. Within population genetic variation was examin...

This study aimed to investigate the contribution of four common DFNB (“DFN” for deafness and “B” for autosomal resessive locus) loci and GJB2 gene mutations (exon 2) in hearing impairment in individuals living in Markazi and Qom provinces of Iran. Forty consanguineous Iranian families with at least three affected individuals in family or pedigree who suffer from an autosomal recessive non-syndr...

Background and Objective: Phenylketonuria (PKU) is a metabolic disorder that is caused by mutations in the phenylalanine hydroxylase (PAH) gene. The multiplicity of mutations in the PAH gene of PKU leads to the cases, in which the pathogenic mutation cannot be detected. In these cases, the variable number of tandem repeat (VNTR), which is the polymorphic marker associated with the PAH gene, is ...

in this study a set of 10 polymorphic microsatellites (maf33, oarcp34, bm8125, maf214, maf70, dyms1, mcm527, oarjmp29, oarjmp58, bm1824) has been used to assess the distribution of genetic variation in six iranian sheep breeds (sanjabi, kabude shiraz, turki ghashghaii, lori, bakhtiyari and arabi). from each breed 45 sheep randomly were selected and their blood samples were collected individuall...

Somatic DNA alterations are known to occur in atherosclerotic carotid artery lesions; however, their significance is unknown. The accumulation of microsatellite mutations in coding DNA regions may reflect a deficiency of the DNA mismatch repair (MMR) system. Alternatively, accumulation of these coding microsatellite mutations may indicate that they contribute to the pathology. To discriminate b...

High-throughput sequencing has been dramatically accelerating the discovery of microsatellite markers (also known as Simple Sequence Repeats). Both 454 and Illumina reads have been used directly in microsatellite discovery and primer design (the "Seq-to-SSR" approach). However, constraints of this approach include: 1) many microsatellite-containing reads do not have sufficient flanking sequence...

Sampling was done using 90 post larvae which were produced by reproduction of some broodstocks of Fenneropenaeus indicus in one day and reared in the same situation for 4 months. Samples were divided into 3 groups: high, medium and low growth (based on weight and length). Genomic DNA was extracted from muscle tissue using the phenol-chloroform method. The polymerase chain reaction (PCR) was car...