The post-transcriptional process of mRNA editing changes up to three amino acids in the second intracellular domain (i2) of the serotonin(2C) (5-HT(2C)) receptor and alters some signaling characteristics of the receptor. Here, we report that the substitution of valine for isoleucine (I156V; 5-HT(2C-VNI)), which occurs naturally as a result of mRNA editing, alters both ligand-dependent and -inde...

Sixty-six specimens of the earthworm Drawida ghilarovi Gates, 1969 (Oligochaeta, Moniligastridae) from 15 localities of the southern Russian Far East were studied cytogenetically. We examined chromosome sets during mitosis and diakinesis as well as DNA content in the spermatogenous and somatic cell nuclei. The populations and morphs displayed no differences in karyotype and ploidy levels estima...

The aim of the study was to determine chromosome number and nuclear DNA content an endemic plant, V. scamandri, using 14-week-old plants germinated from seed genetic stability based on flow cytometry analyses in callus tissue induced MS medium containing 1 mg/L Kin + 2,4-D 9-week-old propagated plants, developed 2 0.1 2,4-D. In mitotic counts showed scamandri had 2n = 16. Flow cytometric analys...

Ascidian early embryonic cells undergo cell differentiation without cell cleavage, thus enabling mixture of cell fate determinants in single cells, which will not be possible in mammalian systems. Either cell in a two-cell embryo (2C cell) has multiple fates and develops into any cell types in a tadpole. To find the condition for controlled induction of a specific cell type, cleavage-arrested c...

cancer is a major global problem and is the second leading cause of mortality in the developed countries.resistance to current chemotherapeutics and high incidence of adverse effects are the two principal reasons for developing new anticancer agents. phenylacetamide derivatives can act as potential anticancer agents. synthesis and screening of 2-(4-fluorophenyl)-n-phenylacetamide derivatives in...

It is well known that DNA-ploidy is useful independent prognosticator of malignancy. However, the biological significance of polyploid cells and the relation between polyploidy and prognosis is not well understood. We analyzed DNA ploidy by flow cytometry in Meth-A cells (a cultured sarcoma cell line) after treatment with K252a, a protein kinase inhibitor, and showed induction of polyploidizati...

Transcripts encoding 5-HT(2C) receptors are modified posttranscriptionally by RNA editing, generating up to 24 protein isoforms. In recombinant cells, the fully edited isoform, 5-HT(2C-VGV), exhibits blunted G-protein coupling and reduced constitutive activity. The present studies examine the signal transduction properties of 5-HT(2C-VGV) receptors in brain to determine the in vivo consequences...

A revertant was isolated from a temperature-sensitive poliovirus 2C mutant, 2C-31, which is defective in viral RNA synthesis. This revertant, called 2C-31R1, grew well at 39 degrees C and was not defective in RNA synthesis. However, in contrast to its parental mutant, 2C-31R1 was cold sensitive and could hardly grow at all at 32 degrees C. Analysis of a single-cycle growth revealed that 2C-31R1...

The Balkan Peninsula is considered an important centre of native tulip species. Tulipa kosovarica and luanica are new species recently discovered in Kosovo, albanica Albania. current study aims at the investigating nuclear DNA content chromosome number these three tulipa order to provide for first time data on their genome size differences among Analysis was performed by flow cytometer (Partec ...

Salvia is a genetically diverse genus in the Lamiaceae family, with hundreds of species distributed globally. With base chromosome numbers ranging from 6 to 19 and ploidy levels diploid octoploid, has been proposed be subdivided based on molecular data rather than morphology. However, little known about total DNA content across genus. The 141 genotypes were analyzed using flow cytometry. Sample...