Genetic data indicate that abrogation of Notch-Rbpj or Wnt-β-catenin pathways results in the loss of the intestinal stem cells (ISCs). However, whether the effect of Notch is direct or due to the aberrant differentiation of the transit-amplifying cells into post-mitotic goblet cells is unknown. To address this issue,we havegenerated composite tamoxifen-inducible intestine-specific genetic mouse...

Evans, Jodi F., James K. Yeh, and John F. Aloia. Osteoblast-like cells of the hypophysectomized rat: a model of aberrant osteoblast development. Am J Physiol Endocrinol Metab 278: E832–E838, 2000.—In a previous work, we demonstrated that the osteoprogenitors derived from the marrow stroma of the hypophysectomized (HX) rat demonstrate enhanced proliferative and differentiation capacities when pl...

Haisch, M., N. K. Fukagawa, and D. E. Matthews. Oxidation of glutamine by the splanchnic bed in humans. Am J Physiol Endocrinol Metab 278: E593–E602, 2000.—[1,2C2]glutamine and [ring-H5]phenylalanine were infused for 7 h into five postabsorptive healthy subjects on two occasions. On one occasion, the tracers were infused intravenously for 3.5 h and then by a nasogastric tube for 3.5 h. The orde...

Goldfarb, David S., William S. Sly, Abdul Waheed, and Alan N. Charney. Acid-base effects on electrolyte transport in CA II-deficient mouse colon. Am. J. Physiol. Gastrointest. Liver Physiol. 278: G409–G415, 2000.—To determine the role of carbonic anhydrase (CA) in colonic electrolyte transport, we studied Car-20 mice, mutants deficient in cytosolic CA II. Ion fluxes were measured under short-ci...

MARVIN REID,1,2 ASHA BADALOO,2 TERRENCE FORRESTER,2 JOHN F. MORLESE,1,2 MARGARET FRAZER,1 WILLIAM C. HEIRD,1 AND FAROOK JAHOOR1 1United States Department of Agriculture/Agricultural Research Service, Children’s Nutrition Research Center, Department of Pediatrics, Baylor College of Medicine, Houston, Texas 77030; and 2Tropical Metabolism Research Unit, University of the West Indies, Mona, Kingst...

Epple, H. J., S. Amasheh, J. Mankertz, M. Goltz, J. D. Schulzke, and M. Fromm. Early aldosterone effect in distal colon by transcriptional regulation of ENaC subunits. Am J Physiol Gastrointest Liver Physiol 278: G718–G724, 2000.— Aldosterone-induced sodium absorption is mediated by the epithelial Na1 channel (ENaC). It is thought that the ‘‘early effect’’ is not based on genomic regulation of ...

Hanani, M., M. Francke, W. Härtig, J. Grosche, A. Reichenbach, and T. Pannicke. Patch-clamp study of neurons and glial cells in isolated myenteric ganglia. Am J Physiol Gastrointest Liver Physiol 278: G644–G651, 2000.— Most of the physiological information on the enteric nervous system has been obtained from studies on preparations of the myenteric ganglia attached to the longitudinal muscle la...

Starritt, Emma C., Richard A. Howlett, George J. F. Heigenhauser, and Lawrence L. Spriet. Sensitivity of CPT I to malonyl-CoA in trained and untrained human skeletal muscle. Am. J. Physiol. Endocrinol. Metab. 278: E462–E468, 2000.—The present study examined the sensitivity of carnitine palmitoyltransferase I (CPT I) activity to its inhibitor malonyl-CoA (M-CoA), and simulated metabolic conditio...