Subchloroplast particles were prepared by incubation with digitonin and differential centrifugation. Such particles show a high rate of cyclic photophosphorylation but have little or no capacity to form the reversible, light-dependent pH gradient. They also have no activity in the formation of the nonphosphorylated high energy intermediate, in acid-base phosphorylation, and in light-induced abs...

Subchloroplast particles were prepared by incubation with digitonin and differential centrifugation. Such particles show a high rate of cyclic photophosphorylation but have little or no capacity to form the reversible, light-dependent pH gradient. They also have no activity in the formation of the nonphosphorylated high energy intermediate, in acid-base phosphorylation, and in light-induced abs...

TP2 was purified from rat testes employing a gentle method involving differential salt extraction of the sonication-resistant spermatid nuclei. The nucleic acid binding properties of TP2 were studied by fluorescence quenching, thermal denaturation, circular dichroism techniques and compared with those of TP1 (Singh, J., and Rao, M. R. S. (1987) J. Biol. Chem. 262, 734-740). The tyrosine fluores...

Possible mechanisms of dihydroxy bile salt-induced K+ secretion by the mammalian colon were evaluated by studying the effects of taurochenodeoxycholate (TCDC) on 86Rb+ transport across the isolated, short-circuited rabbit distal colon. Simultaneous measurements of 86Rb+ and 42K+ unidirectional fluxes were highly correlated [r = 0.964 for serosal (s) to mucosal (m) and 0.765 for m to s], indicat...

In this study, locally produced Metakaolin (MK) was used as an admixture in recycled aggregate concrete of grades M 25 and 30. The content MK varied from 0-15% at 5% intervals. physical mechanical properties (bulk density, specific gravity, water absorption, crushing value impact value) aggregates were determined, the chemical composition well reactivity evaluated using X-Ray Fluorescence (XRF)...

Adenosine (10(-7)-3 x 10(-4) M) produced concentration-dependent and endothelium-independent relaxation of isolated rat inferior mesenteric artery. Application of indomethacin (10(-5) M) or N(G)-nitro-L-arginine (10(-5) M) did did not alter adenosine-elicited relaxation. Conversely, in the presence of high concentration of K+ (100 mM), ouabain (10(-4)) or combination of tetraethylammonium (5 x ...

A simple and rapid method for determination of mexiletine in pharmaceutical preparations was developed and validated using gas chromatography with flame ionization detection (GC-FID). GC separation was performed in about 5 min using a HP-5 capilary column (30 m x 0.320 mm, 0.25μm). Nitrogen was used as carrier gas at a flow-rate of 2 mL min. After injection of the sample at inlet temperature 25...

The association-dissociation kinetics of Escherichia coli MRE 600 “tight” (Type A) ribosomes (30 S + 50 S e 70 S ) for a variety of conditions have been studied in a light-scattering stopped-flow apparatus. The rate constants for the M&’-induced subunit association and 70 S dissociation reactions were 1.7 X lo’ M” s-’ (18 n m M&’) and 0.015 s-l (1 m M@’), respectively. The addition of ribosomal...

BACKGROUND Traditional Bankart repair using bone tunnels has a reported failure rate between 0% and 5% in long-term studies. Arthroscopic Bankart repair using suture anchors has become more popular; however, reported failure rates have been cited between 4% and 18%. There have been no satisfactory explanations for the differences in these outcomes. HYPOTHESIS Bone tunnels will provide increas...

The association-dissociation kinetics of Escherichia coli MRE 600 “tight” (Type A) ribosomes (30 S + 50 S e 70 S ) for a variety of conditions have been studied in a light-scattering stopped-flow apparatus. The rate constants for the M&’-induced subunit association and 70 S dissociation reactions were 1.7 X lo’ M” s-’ (18 n m M&’) and 0.015 s-l (1 m M@’), respectively. The addition of ribosomal...