Second-strand cDNA priming is a central problem for full-length characterization of transcripts. A new strategy using bacteriophage T4 DNA ligase and partially degenerate adapters is proposed for grafting a sequence tag to the end of polyribonucleotides. Based on this RNA tagging system and previously described protocols, a new method for full-length cDNA production has been implemented. Valida...

Oral supplementation with potassium bromide (10, 20 and 40 ?g/ml) to fifth instar larvae of CSR2, CSR4 and CSR2xCSR4 crossbreed races of the silkworm, Bombyx mori resulted in a significant increase in the fat body glycogen in all the treated groups and in all the three races of the silkworm, B. mori. The fat body protein content was increased with 20 and 40 ?g/ml treated groups in CSR2 and CSR4...

3′ Rapid amplification of cDNA ends (RACE) has been an invaluable tool for molecular biologists since its inception (1). This method and the subsequent method of 3′ RACE walking (2) enable the simple and effective amplification of 3′ flanking cDNA sequence in a ligation-independent manner (3). However, despite their capacity for the suppression of nonspecific PCR products (4), they remain essen...

Collection of racial data is ubiquitous throughout research as an important measure of the demographic characteristics of the study population. However, the validity of racial data has been a concern, prompting several agencies to modify their measurements by allowing individuals to identify with multiple racial categories. This research aims to add to the current methodology for analyzing mult...

A simple, efficient and sensitive RACE-based procedure was developed for the determination of unknown 5' regions from bacterial cDNA. A number of critical modifications were made to the standard RACE method, including the optimization of the RNA extraction, reverse transcription and PCR conditions. This procedure was used to accurately determine the site of transcript initiation and structure o...

The host-parasite relationships of asparagus and Meloidogyne spp. were examined under greenhouse and microplot conditions. Meloidogyne species and races differed greatly in their ability to reproduce on asparagus seedlings. Meloidogyne hapla generally failed to reproduce, and M. javanica, M. arenaria race 1, and M. incognita race 3 reproduced poorly, with a reproduction factor (Rf = final popul...

purpose the aim of this study was to investigate the relation between anthropometric variables and total race time including split times in 184 recreational male ironman triathletes. methods body mass, body height, body mass index, lengths and circumferences of imbs, thicknesses of skin-folds, sum of skin-fold thicknesses, and percent body fat were related to total race time including split tim...

Racial differences on mental test scores are some of the most well-documented findings in the study of individual differences, yet only in the past 10 years have neuropsychologists undertaken serious examination of ethnic and related demographic concomitants of neuropsychological test performance. The present study examined performance differences for blacks and whites on 14 separate measures o...

RACE [3] is a successor of HAM-ALC [5, 2]. Based on sound and complete algorithms RACE currently implements TBox and ABox reasoning for the description logic ALCNHR+ [4] that supports number restrictions, role hierarchies, and transitively closed roles. Note that this DL implies general concept inclusions as a language feature. RACE accepts TBoxes and ABoxes in the KRSS syntax with appropriate ...