Three Japanese individuals with homozygous ‘-thalassemia from different families were the subjects of molecular genetic analysis. They were homozygous for seven polymorphic sites in the fl-globin gene cluster. Nucleotide sequence analysis of the 6-globin gene cloned from each patient revealed a single nucleotide substitution (T-C) 77 base pairs 5’ to the cap site, just upstream of the CCAAC box...

ST-246 (Tecovirimat) is a small synthetic antiviral compound being developed to treat pathogenic orthopoxvirus infections of humans. The compound was discovered as part of a high throughput screen designed to identify inhibitors of vaccinia virus-induced cytopathic effects. The antiviral activity is specific for orthopoxviruses and the compound does not inhibit the replication of other RNA- and...

Dystrophin has been shown to be associated in cells with actin bundles. Dys-246, an N-terminal recombinant protein encoding the first 246 residues of dystrophin, includes two calponin-homology (CH) domains, and is similar to a large class of F-actin cross-linking proteins including alpha-actinin, fimbrin, and spectrin. It has been shown that expression or microinjection of amino-terminal fragme...

The general overexpression of p53 by different types of tumor cells suggests that p53 immunity might be generally useful for tumor immunotherapy. We describe here the induction of immunity to p53 and resistance to tumor metastasis using an idiotypic network. Mice were immunized with domain-specific anti-p53 monoclonal antibodies (Ab1): PAb-248 directed to the N-terminus; PAb-246 directed to the...

This report discusses the design of computational experiments to test heuristic methods and provides reporting guidelines for such experimentation. The goal is to promote thoughtful, well-planned, and extensive testing of heuristics, full disclosure of experimental conditions, and integrity in and reproducibility of the reported results. Departmentof Computer Science andEngineering, SouthernMet...

We present a superpixel method for full spatial phase and amplitude control of a light beam using a digital micromirror device (DMD) combined with a spatial filter. We combine square regions of nearby micromirrors into superpixels by low pass filtering in a Fourier plane of the DMD. At each superpixel we are able to independently modulate the phase and the amplitude of light, while retaining a ...

A total of 101 Campylobacter isolates from Nigerian children with or without gastroenteritis were biotyped and serogrouped by using the Lior typing schemes (H. Lior, J. Clin. Microbiol. 20:636-640, 1984; H. Lior, D. L. Woodward, J. A. Edgar, L. J. Laroche, and P. Gill, J. Clin. Microbiol. 15:761-768, 1982). Fifty-three (52.5%) of the isolates were Campylobacter jejuni biotype I, 29 (28.7%) were...

Endometriosis is a complex disease involving multiple susceptibility genes and environmental factors. Our previous studies on endometriosis identified a region of significant linkage on chromosome 10q. Two biological candidate genes (CYP17A1 and IFIT1) located on chromosome 10q, have previously been implicated in endometriosis and/or uterine function. We hypothesized that variation in CYP17A1 a...

A rapid, inexpensive, simple, and accurate multiplex polymerase chain reaction (PCR) was developed in a single tube for identification of Mycobacterium tuberculosis. Assessment of sensitivity and specificity of simple PCR was performed with 116 strains of M. tuberculosis complex (MTC) and 144 strains of nontuberculous mycobacteria (NTM) compared with the biochemical method. Specific amplificati...