Introduction The micro heterogeneity or quality of a protein has been shown to have a significant impact on its physical, chemical and biological properties both in vitro and in vivo [1]. Micro heterogeneity is evaluated in terms of post translational modifications such as glycosylation, charge variants, aggregates and fragments profile. The biggest challenge in process development is to find a...

purpose: the aim of this study was to compare the effects of a) a flame heated endo plugger, b) gatesglidden drills and c) gpx drills on the apical seal for the purpose of post space preparation. materials & methods: the crowns of sixty six extracted maxillary central teeth were removed from cej by a diamond disc and the root canals were prepared and obturated with guttapercha and sealer (ah26)...

background post-term pregnancy is an important factor in perinatal mortality and morbidity. generally, to reduce perinatal mortality in pregnancy, the delivery is done before adverse perinatal morbidity occurs. to prevent prolonged pregnancy, labor is induced with chemical drugs and complementary therapies. due to the side effects and contraindications of chemical medicine, the use of herbs has...

purpose: to report a case who had undergone lasik in his right eye and prk in the fellow eye for correction of equivalent myopia but developed progressive post-operative keratectasia only in the right eye. patient and findings: a 30-year-old man had undergone lasik in right eye 3 years ago for correction of refractive error of -1.75  -1.50@48° and prk in left eye 2 years ago for correction of r...

Many DNA lesions cause pausing of replication forks at lesion sites thus generating gaps in the daughter-strands that are filled-in by post replication repair (PRR) pathways. In S. cerevisiae, PRR involves Translesion Synthesis (TLS) mediated by Polη or Polζ, or Rad5-dependent gap-filling via a poorly characterized error-free mechanism. We have developed an assay to monitor error-free and mutag...

Allyl sulfides undergo efficient cross-metathesis in aqueous media with Hoveyda-Grubbs second generation catalyst 1. The high reactivity of allyl sulfides in cross-metathesis was exploited in the first examples of cross-metathesis on a protein surface. S-Allylcysteine was incorporated chemically into the protein, providing the requisite allyl sulfide handle. Preliminary efforts to genetically i...

This prospective, Post-Authorization Safety Surveillance (PASS) study was carried out in patients with hemophilia A or B and inhibitors treated with FEIBA for 1 year to collect real-world data on safety and effectiveness of FEIBA. The study followed a cohort design and did not make stipulations on treatment or observation schedule, as it was designed to observe routine medical practices based o...

In this paper, we study how to analyze and improve the quality of a large historical newspaper collection. The National Library of Finland has digitized millions of newspaper pages. The quality of the outcome of the OCR process is limited especially with regard to the oldest parts of the collection. Approaches such as crowdsourcing has been used in this field to improve the quality of the texts...

Poly ADP-ribose polymerases (PARPs) catalyze massive protein poly ADP-ribosylation (PARylation) within seconds after the induction of DNA single- or double-strand breaks. PARylation occurs at or near the sites of DNA damage and promotes the recruitment of DNA repair factors via their poly ADP-ribose (PAR) binding domains. Several novel PAR-binding domains have been recently identified. Here, we...

Protein phosphorylation is a posttranslational modification (PTM or PTLM), where a phosphoryl group is added to the residue(s) of a protein molecule. The most commonly phosphorylated amino acids occur at serine (S), threonine (T), and tyrosine (Y). Protein phosphorylation plays a significant role in a wide range of cellular processes; meanwhile its dysregulation is also involved with many disea...