Cytochrome P4501IEI is responsible for the activation of carcinogenic N-nitrosamines, benzene, urethane, and other low-molecularweight compounds. Restriction fragment length polymorphisms (Pstl and RsaI restriction enzymes) have been identified in the cytochrome P4501IE1 transcription regulatory region that may affect expression. This study describes the PstI and RsaI polymorphisms in different...

SOURCE AND DESCRIPTION OF CLONE: 0.6 kb anonymous lymphocyte cDNA cloned into the PstI site of pUR291. POLYMORPHISM: Bglll identifies a two allele polymorphism with a band at either 5.2 kb (A 1) or 5.0 kb (A 2) and invariant bands at approximately

SOURCE/DESCRIPTION; A 2.0 kb Mspl fragment from cosmid YNH24 isolated by HBV-2 oligonucleotide (GGAGTTGGGGGAGGAG) (1) was subcloned into the AccI site of pUC18. POLYMORPHISM: Mspl identifies a >30 allele VNTR polymorphism with bands between 1.0-5.0 kb. Taql, Bglll, Pvull, PstI and BamHI also detect the same polymorphism.

Plasmid pUA466, a 45-kilobase transmissible tetracycline resistance plasmid from Campylobacter jejuni was mapped with AvaI, AvaII, BclI, HincII, PstI, XhoI, and XbaI. The resistance determinant was cloned and expressed in Escherichia coli and was homologous with a class M determinant from Streptococcus spp.

DNA from the Streptomyces plasmid pIJ702 was mutagenized in vitro using hydroxylamine and transformed into Streptomyces lividans. One plasmid with temperature-sensitive replication (pMT660) and one plasmid with a temperature-sensitive tyrosinase (pMT661) were isolated. The plasmid pMT661 contains a novel PstI restriction endonuclease site within the tyrosinase gene.

The complete sequence of plasmid pEA29 from Erwinia amylovora strain Ea88 consists of 28,185 bp with a 50.2% G+C content. As deletions and insertions were detected in other derivatives of pEA29, its size actually varied from 27.6 to 34.9 kb. Thirteen open reading frames that encoded predicted proteins with similarities to known proteins from other bacteria were identified along with two open re...

The gene coding for xylulokinase has been isolated from the yeast Pachysolen tannophilus by complementation of Escherichia coli xylulokinase (xylB) mutants. Through subcloning, the gene has been localized at one end of a 3.2-kilobase EcoRI-PstI fragment. Expression of the cloned gene was insensitive to glucose inhibition. Furthermore, the cloned gene did not cross-hybridize with E. coli and Sac...

The gene encoding the outer membrane phosphate-selective porin protein P from Pseudomonas aeruginosa was cloned into Escherichia coli. The protein product was expressed and transported to the outer membrane of an E. coli phoE mutant and assembled into functional trimers. Expression of a product of the correct molecular weight was confirmed by sodium dodecyl sulfate-polyacrylamide gel electropho...