background: mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. this study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection procedures. methods: in the current study, calcium phosphate (cap), deae-...

many cell lines, when cultured under proper conditions, can form three dimensionalstructures called multicellular spheroids. these spheroids resemble in vivo tumor models in severalaspects. therefore, studying growth characteristics and behavior of spheroids is beneficial inunderstanding the behavior of tumors under various experimental conditions. in this work, we havestudied the growth proper...

This research aims to study the effect of interference with tripartite motif-containing protein 26 expression on proliferation intracranial glioma cells. randomly selected 60 cases specimens and brain tissue 10 trauma patients as a normal control group. First, cell culture is performed observe growth status Subsequently, small interfering ribonucleic acid technique was used interfere lines A172...

BACKGROUND Resistance to chemotherapy is a growing concern, thus natural anticancer agents are drawing the attention of many scientists and clinicians. One natural anticancer agent, umbelliprenin, is a coumarin produced by many species of Ferula. OBJECTIVES We aimed to examine the inhibitory effect of umbelliprenin on human and mouse bone marrow-derived stem cells (BMDSCs), peripheral blood m...

Abstract ONC201 is the first bitopic dopamine receptor D2 (DRD2) antagonist and allosteric mitochondrial protease ClpP agonist. well tolerated induces durable tumor regressions in H3 K27M-mutant glioma patients. ONC206, a derivative of currently Phase I trials, also DRD2 agonist with enhanced nanomolar potency relative to ONC201. We evaluated vitro efficacy, mechanism(s) action acquired resista...

c) The number of text pages, number of tables, figures, and references, and the number of words in the Abstract, Introduction and Discussion (each item should be placed on a separate line) Text pages: 33 Number of tables: 1 Number of figures: 8 Number of references: 40 Number of words in the Abstract: 249 Number of words in the Introduction: 669 Number of words in the Discussion: 1 484 d) A lis...

The platelet-derived growth factor (PDGF) A-chain gene is expressed in a tissue- and developmental stage-specific manner. Here we identify an S1 nuclease sensitive region within the first intron that functions as a negative regulatory element in HeLa but not in human glioblastoma (A172) cells in transient transfection assays. A 147 bp DNA fragment that contains this element functions in a posit...

Glioblastoma (GBM) is one of the most lethal forms of human cancer, and new clinical biomarkers and therapeutic targets are urgently required. microRNAs (miRNAs) are small, non-coding RNAs that negatively regulate gene expression at the post-transcriptional and/or translational level by binding the 3' untranslated regions (3' UTRs) of target mRNAs. The dysregulated expression of several miRNAs ...

Sodium butyrate (SB), a short chain (C-4) saturated fatty acid, is present in the human bowel at increased concentrations (~2 mM) as a food metabolite. It has been demonstrated that SB exerts an anti-tumor effect as a histone deacetylase inhibitor; however, its precise mechanism of action remains to be elucidated. The present study focused on the mechanisms underlying the effects of SB on gliob...

the lung cancer is one of the most dangerous cancers and is also the leading cause of cancer death worldwide, accounting for about 1.3 million deaths annually. however in clinical practice, lung cancer therapies commonly do with chemotherapy, although it is hard because the lung cancer may progress to metastasis stage. the metastasis of lung cancer is highly dependent of expression of matrix me...