The effects of two hypolipidemic agents, 2-methyl-2-[p(1,2,3,4-tetrahydro-l-naphthyl)-phenoxy]propionate and ethyl 2-(p-chlorophenyl)-2-methylpropionate, on the activity of a highly purified preparation of acetyl coenxyme A carboxylase, obtained from chicken livers, were studied. Both compounds are inhibitors of acetyl-CoA carboxylase, but they have no inhibitory effect on a number of other enz...

Acetyl-coenzyme A (acetyl-CoA) formed within the plastid is the precursor for the biosynthesis of fatty acids and, through them, a range of important biomolecules. The source of acetyl-CoA in the plastid is not known, but two enzymes are thought to be involved: acetyl-CoA synthetase and plastidic pyruvate dehydrogenase. To determine the importance of these two enzymes in synthesizing acetyl-CoA...

Studies presented here, with the criterion of the degree of binding of the biotin derivatives obtained from acetyl coenzyme A carboxylase to avidin, show that these compounds have an intact ureido ring, eliminating the diamine derivative as an intermediate in the carboxylation. With the use of carboxyl-14C-biotin acetyland propionyl-CoA carboxylases, it has been possible to show both in vivo an...

Acetyl-CoA carboxylase (E.C. 6.4.1.2) was isolated from rat liver. The purified enzyme contains phospholipids with a rather large amount of phosphatidylinositol (26%). Incubation of the purified acetyl-CoA carboxylase with phospholipase A2 (E.C. 3.1.1.4) or with phospholipase D (E.C. 3.1.1.4) diminishes the phospholipid content by 70%, this treatment leading to a complete inactivation of the en...

Malonyl-coenzyme A (CoA) is a crucial extender unit for the synthesis of mycolic and other fatty acids in mycobacteria, generated in a reaction catalyzed by acetyl-CoA carboxylase. We previously reported on the essentiality of accD6Mtb encoding the functional acetyl-CoA carboxylase subunit in Mycobacterium tuberculosis. Strikingly, the homologous gene in the fast-growing, non-pathogenic Mycobac...

The main lipogenic tissues of the rat are the liver, white adipose tissue and, during lactation, the mammary gland. The rate of fatty acid synthesis de novo in these tissues must be regulated according to the nutritional and physiological status of the animal. Such short-term regulation is primarily achieved through the action of hormones. Acetyl-CoA carboxyulase is a prime target for such horm...

Previous reports from this laboratory (R. K. Rasmussen and H. P. Klein, Biochem. Biophys. Res. Commun. 28:415, 1967) indicated that many organic compounds can stimulate the acetylcoenzymeA (CoA) carboxylase of Saccharomyces cerevisiae strain LK2G12. The enzyme from this organism appears to differ from that reported by Matsuhashi et al. (Biochem. Z. 340:243, 1964; Biochem. Z. 340:263, 1964), who...