The present study was carried out for the detection and identification of bovine tuberculosis (bTB) using polymerase chain reaction (PCR). A total of 10 suspected cattle of Savar and BAU dairy farm were examined. Lymphnode biopsy, nasal swabs and blood were collected. Smears from lymphnode biopsy and nasal swabs were made onto clear slides and stain with acid fast staining. Portion of lymohnode...

Skin biopsy specimens from 3 consecutive patients with lesions of Kaposi's sarcoma limited to the skin were cultured for bacteria, and also examined histopathologically for the presence of acid-fast wall deficient (CWD) bacteria. Corynebacterium sp and Propionibacterium acnes were isolated from two repeated cultures from the first case. Both Staphylococcus epidermidis and Streptococcus viridans...

The objective of the present study was to analyze the frequency of oocysts of Cryptosporidium spp in fecal samples from children aged one to fourteen years at a public daycare center located in a needy community in the city of Recife, Pernambuco. The investigation was carried out between June 28, 2006, and April 3, 2007, and involved 182 children. Among the samples analyzed, 59 (32.4%) were pos...

C. Muratore, V. Varshney, J. J. Gengler, J. J. Hu, J. E. Bultman, T. M. Smith, P. J. Shamberger, B. Qiu, X. Ruan, A. K. Roy, and A. A. Voevodin Department of Chemical and Materials Engineering, University of Dayton, Dayton, Ohio 45469, USA Materials and Manufacturing Directorate, Air Force Research Laboratory, Wright-Patterson AFB, Ohio 45433, USA Universal Technology Corporation, Dayton, Ohio ...

One of the simplest procedures to demonstrate mucinous substances (acid mucopolysaccharides) in routine histological preparations involves the use of Alcian dyes. Methods involving these dyes have largely. replaced the older, more empirical mucicarmine technique arid are faster and less complicated than the colloidal iron method of Hale,1 which suffers from the possibility of false-positive sta...

The use of a liquid culture system such as MGIT broth has greatly improved the sensitivity of isolating mycobacteria in clinical laboratories. Microscopic visualization of acid fast bacilli (AFB) in the culture positive MGIT broth remains the first routine step for rapidly indicating the presence of mycobacteria. We modified an ultraviolet (UV) light fixation process to increase AFB cells adher...

A rapid dimethyl sulfoxide modification of an acid-fast technique was applied to direct fecal smears to monitor cryptosporidiosis in nonhuman primates. Brilliantly stained pink oocysts against a pale green background demonstrated well-preserved internal morphology and facilitated rapid, simple, noninvasive diagnosis without fluorescent or phase-contrast microscopy.

Tuberculous pleural effusion occurs in approximately 5% of patients with Mycobacterium tuberculosis (TB) infection1 and accounts for 4% of all TB cases in the United States.2 Diagnosis is challenging, with 48–96% of tuberculous pleural effusions negative by sputum acid-fast bacilli (AFB) stain and culture. Thoracentesis is frequently performed and shows an exudative, lymphocytic pleural effusio...

The language of partial order time expresses the issues central to many problems in asynchronous distributed systems. A secure partial order time service would provide a general method to develop secure protocols for these problems. In this paper, we sketch out these issues and develop one such protocol: signed vector timestamps. The majority of this paper is drawn verbatim from the rst author'...