Lithium-ion batteries (LIBs) are extensively used for power storage in most gadgets, electric vehicles (EV), and energy devices. Spent LIBs an excellent source of metals, which can be recycled reused new to reduce environmental impacts. Our current study reports bioleaching-mediated metal recovery from spent nickel-, manganese-, cobalt (NMC)-based at a high solid content, using autotrophic bact...

Toxin-antitoxin (TA) systems are genetic modules composed of a pair of genes encoding a stable toxin and an unstable antitoxin that inhibits toxin activity. They are widespread among plasmids and chromosomes of bacteria and archaea. TA systems are known to be involved in the stabilization of plasmids but there is no consensus about the function of chromosomal TA systems. To shed light on the ro...

A 1.3-kb insertion sequence, termed ISAfe1 (U66426), from Acidithiobacillus ferrooxidans ATCC 19859 is described. ISAfe1 exhibits the features of a typical bacterial insertion sequence. It has 26-bp, imperfectly matched, terminal inverted repeats and an open reading frame (ORF) that potentially encodes a transposase (TPase) of 404 amino acids (AAB07489) with significant similarity to members of...

Acidithiobacillus ferrooxidans (A. ferrooxidans, formerly Thiobacillus ferrooxidans) is the Gram-negative, acidophilic, chemolithoautotrophic bacterium that obtains its energy from the oxidation of ferrous iron, elemental sulphur, or partially oxidized sulphur compounds (Leduc and Ferroni, 1994; Rohwerder et al., 2003), even hydrogen or formate in anoxic conditions (Drobner et al., 1990; Pronk ...

Acidithiobacillus ferridurans JAGS is a newly isolated acidophile from an acid mine drainage (AMD). The genome of isolate was sequenced and compared with eight other published genomes Acidithiobacillus. pairwise mutation distance (Mash) average nucleotide identity (ANI) revealed that had close evolutionary relationship A. JCM18981, but whole-genome alignment showed it higher similarity in genom...

Investigations into the cause and control of the production of sulfuric acid in effluents of coal mines, and the effects of such effluents upon streams were begun in 1946.1 Working and abandoned mines of Western Pennsylvania alone contribute an estimated million tons of sulfuric acid per year to the drainage area of the Ohio River. A field survey of mine drainage and receiving streams was inaug...

An antiserum raised against whole cells of Thiobacillus ferrooxidans was allowed to react with a variety of acidophilic and nonacidophilic bacteria in an enzyme-linked immunosorbent assay and an indirect immunofluorescence assay. Both experiments demonstrated that the antiserum was specific at the species level. This preparation was used to evaluate the role of T. ferrooxidans in the microbial ...