The mechanisms involved in fluoroquinolone resistance in Salmonella enterica include target alterations and overexpression of efflux pumps. The present study evaluated the role of known and putative multidrug resistance efflux pumps and mutations in topoisomerase genes among laboratory-selected and naturally occurring fluoroquinolone-resistant Salmonella enterica serovar Typhimurium strains. St...

Drug efflux protein complexes confer multidrug resistance on bacteria by transporting a wide spectrum of structurally diverse antibiotics. Moreover, organisms can only acquire resistance in the presence of an active efflux pump. The substrate range of drug efflux pumps is not limited to antibiotics, but it also includes toxins, dyes, detergents, lipids, and molecules involved in quorum sensing; hence...

background urinary tract infection (uti) is a common problem in infants and children, as well as adults. objectives the aim of this study was to assess the most common bacterial uropathogens, their susceptibility, and resistance to antibiotics in children with uti. materials and methods this study included 7,365 urine samples sent from various departments to the kars state hospital microbiology...

The AcrAB-TolC efflux pump plays an intrinsic role in resistance to hydrophobic solvents in Escherichia coli. E. coli OST5500 is hypersensitive to solvents due to inactivation of the acrB gene by insertion of IS30. Suppressor mutants showing high solvent resistance were isolated from OST5500. These mutants produced high levels of AcrE and AcrF proteins, which were not produced in OST5500, and i...

OBJECTIVES The physiological response of Salmonella enterica serovar Typhimurium to fluoroquinolone antibiotics was investigated using proteomic methods. METHODS Proteomes were prepared from strain SL1344 following treatment of broth cultures with ciprofloxacin (0.03 and 0.008 mg/L; 2x and 0.5x MIC) and enrofloxacin (0.03 mg/L) and from a multiple antibiotic resistant (MAR) mutant. Protein ex...

Immunoblotting with antibody against AcrA, an obligatory component of the AcrAB multidrug efflux system, showed that this protein was overexpressed by >/=170% in 9 of 10 clinical isolates of Esherichia coli with high-level ciprofloxacin resistance (MICs, >/=32 microg/ml) but not in any of the 15 isolates for which the MIC was </=1 microg/ml.

background: the science of identification and employment of medicinal plants dates ‎back to the early days of man on earth. cannabis (hashish) is the most common illegal ‎substance used in the united states and was subjected to extensive research as a ‎powerful local disinfecting agent for mouth cavity and skin and an anti-tubercular agent ‎in 1950.‎ methods: clinical strains were isolated from...

background mercury is one of the most toxic heavy metals. even a small amount of it is toxic for all living organisms. some of bacteria have developed special resistance mechanisms against mercury, in addition to resistant to different antibiotics. these bacteria usually acquire hg and antibiotic resistance genes via horizontal gene transfer in their habitat. objectives the aim of this study wa...

conclusions metronidazole and vancomycin still seem to be most effective drugs for treatment cdi. objectives the aim of this study was to investigate the antimicrobial susceptibility patterns of c. difficile clinical isolates against antibiotics commonly used for treatment cdi in hospitalized patients. material and methods during a 12 month study, 75 c. difficile isolates were collected from 39...