There is a great potential for the development of new cell replacement strategies based on adult human neural stem-like cells. However, little is known about the hierarchy of cells and the unique molecular properties of stem- and progenitor cells of the nervous system. Stem cells from the adult human brain can be propagated and expanded in vitro as free floating neurospheres that are capable of...

Considering the ease of isolation and high expansion potential of pulp stem/progenitor cells isolated from wisdom and primary teeth they have been implicated as the most reliable autologous cell source in dental tissue engineering. Meanwhile, different isolation methods have remarkable impacts on the expansion potential of adult stem cells. In enzymatic digestion method extracted teeth as denta...

abstract introduction: cancer stem cells are a small subpopulation of cells within a tumor which are responsible for maintaining the tumor mass. a number of factors such as oct-4 that govern the fate of adult stem cells also play a role in malignant cell transformation. oct-4 is a key regulator of self-renewal in embryonic stem cells; its expression is potentially correlated with tumorigenesis ...

Ex vivo expansion of human umbilical cord blood cells (HUCBC) is explored by several investigators to enhance the repopulating potential of HUCBC. The proliferation and expansion of human hematopoietic stem cells (HSC) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding HSC for patient transplantation. Using primary human mesenchymal stem ...

Stem cells can self-renew and maintain the ability to differentiate into mature lineages. Whereas the ‘‘stemness’’ of embryonic stem cells is not discussed, the primitiveness of a stem cell type within adult organisms is not well determined. Data presently available are either inconclusive or controversial regarding two main topics: maintenance or senescente of the adult stem cell pool; and plu...

received: 23/sep/2008, accepted: 23/nov/2008oct-4/ pou5f1/ oct-3, a pou domain family protein acts as a crucial transcription factor dur- ing embryonic development. it helps in maintenance of self renewal as well as pluripotential state of embryonic stem (es) cells. its expression starts right from 2 cell stage especially prior to 8 cell stage till the blastocyst stage where it is strongly exp...

The adult mouse forebrain, which exhibits substantial ongoing cell genesis, contains self-renewing multipotent neural stem cells that respond to epidermal growth factor (EGF), but the adult spinal cord, which exhibits limited cell genesis, does not. Spinal cord development is a process characterized by defined periods of cell histogenesis. Thus, in the present study we asked whether EGF-respons...

background: dendritic cells (dcs) are the most potent stimulators of primary t cell responses and play a key role in immune reactions after stem cell transplantation. very little is known about the cord blood (cb) dendritic cells and their potential involvement in the low incidence and lower severity of acute graft-versus-host disease after cb transplantation.   objectives: the aim of this stud...