RNA synthesis (5, 8, 20), a secondary consequence of inhibition of RNA polymerase activity (6, 9, 10). The present experiments were undertaken to provide further information on several aspects of hepatocyte nuclear responses. Specifically, earlier studies have, for the most part, involved observations made at one or a few fixed time intervals following toxin treatment. Also, they have often bee...

The effectiveness of microwave heating has been evaluated for the detoxification aflatoxins contaminated peanut and products. products comprise various confectionery such as brittle toffee, slabs sugar jaggery, roasted salted butter which were highly with B1 ranging from 5 to 183µg/kg aflatoxin B2 7 46.7µg/kg. level was determined subsequently treated heating, resulted in reduction content. coo...

BACKGROUND AND PURPOSE Mycotoxins are secondary fungal metabolites with a very high diversity that are produced by some species of Aspergillus which frequently leads to contaminate food and agricultural products. Recently, elimination of aflatoxin contamination in food and feed has been considered by scientists worldwide. Although, the antibacterial and antifungal effects of vitamins as natural...

Whole-body autoradiography of 3H-labeled aflatoxin B1 in young pigs showed a localization of bound label in the nasal olfactory and respiratory mucosa, in the tracheo-laryngeal mucosa, and in the conjunctiva, in addition to the liver. Whole-body and microautoradiography also showed a labeling of pigmented tissues, which can be ascribed to a melanin binding of AFB1. In vitro experiments with mic...

Activation of ras proto-oncogenes occurs frequently in vivo in chemically induced rodent tumours, including rat hepatomas induced by aflatoxin B1. This study examines the in vitro activation of a human ras gene by this mycotoxin. A plasmid containing the human Ha-ras proto-oncogene, together with a neomycin resistance gene (pECneo), was incubated in vitro with a microsomal system generating afl...

Plants are unique sources of useful metabolites. Plant essential oils display a wide range of antimicrobial effects against various pathogens. Here, we studied the essential oil from the seeds of Carum copticum. We monitored aflatoxin by high-performance liquid chromatography. Results show that Carum copticum essential oil inhibits Asergillus parasiticus growth and prevents aflatoxin production...

An improved monoclonal antibody immunoaffinity chromatography/high-pressure liquid chromatography/ fluorescence detection method was developed to measure aflatoxin (AF) exposure by quantifying AFM1 in human and rat urine samples. Analysis of different amounts of various AF metabolites showed that the immunoaffinity resin was highly selective for aflatoxin B1 (AFB1), AFB2, and AFM1. Recovery of ...

Photochemical detoxification reactions have been used to generate photooxidised snake venom product of 0 pharmacologic and therapeutic significance. Photochemical method by exposure to UV radiation for120 min. at 30 C in the presence of methylene blue was used to generate photooxidised aflatoxin B1 product (POAFB1). TLC revealed three spots under UV light illumination and HPLC analysis showed t...

Aflatoxins, metabolites of the fungi Aspergillus flavus and Aspergillus parasiticus, are frequent contaminants of a number of staple foods, particularly maize and ground nuts, in subsistence farming communities in tropical and sub-tropical climates in sub-Saharan Africa, Eastern Asia and parts of South America. Contamination of foods occurs during growth and as a result of storage in deficient ...