In order to assess the prognostic significance of silver-stained nucleolar organizer region (AgNOR) proteins, a standardised analysis has been performed on 34 ocular globes with choroidal melanomas. On formalin-fixed paraffin-embedded sections, the visualisation and quantification of AgNORs were achieved according to the guidelines of the Committee on AgNOR Quantification (1995); statistical an...

Argyrophilic nucleolar organizer region (AgNOR) counts in cytologic and histologic lymph node samples from healthy dogs and dogs with lymphoma were compared. Control samples were taken from 10 Beagle dogs (six female and four male dogs, 1.5-2 years), and lymphoma samples were taken from 16 dogs. Cytologic samples were obtained by fine-needle aspiration and impression and histologic samples by e...

BACKGROUND: Cyclooxygenase-2 (COX)-2 inhibitor (Celecoxib) is a non-steroidal antiinflammatory drug (NSAIDs) and over-expression of COX-2 protein and mRNA has been reported in various cancer tissues. Therefore, it has been suggested that COX-2 is related to carcinogenesis. METHODS: Twenty five albino rats were used .They were divided into 3 groups; group I (normal control) and group II and III ...

Settings. Despite the limited diagnostic utility of AgNORs (argyrophilic nucleolar organiser region-associated proteins) for individual breast lesions, AgNOR analysis bears a significant potential for characterizing cell proliferative activity of breast lesions. Methodology. The present study investigated the relationship between mean AgNORs count and immunohistochemical expression of ER, PR, H...

The family Prochilodontidae is considered a group with well conserved chromosomes characterized by their number, morphology and banding patterns. Thence, our study aimed at accomplishing a cytogenetic analysis with conventional methods (Giemsa staining, silver staining of the nucleolus organizer regions-AgNOR, and C-banding) and fluorescence in situ hybridization (FISH) with 18S and 5S ribosoma...

One of the most important questions in clinical routine is to find out patients with good or worse prognosis to apply an optimal therapy scheme for each patient. In this study 58 patients with different neuroendocrine tumours of the lung were investigated. Histological sections were prepared with different stainings (MIB-1, AgNOR, Feulgen). By means of high resolution image cytometry stereologi...

Using a silver staining technique, nucleolar organiser regions were identified in routinely processed paraffin sections of a range of dermal melanotic lesions. The technique shows argyrophilic NOR associated proteins (AgNORs), which are seen in nuclei as black dots. Although the nuclei of melanocarcinoma in situ, melanocarcinoma per se, and lentigo maligna contained similar numbers of AgNORs, i...

It has been shown that AgNOR staining can provide useful information for diagnosis in cytology of the oral cavity [22], body fluids [3,18], hematologic smears [10,11,14–17] or lymph node aspiration cytology [12]. We would like draw attention of the readers towards an easy AgNOR evaluation which has shown to be very useful as prognostic marker in chronic lymphoid leukemia (CLL) patients. This di...

Silver binding nucleolar organizer regions (AgNOR) in normal oral mucosa (NOM), oral leukoplakia with epithelial dysplasia (ED), and oral squamous cell carcinoma (OSCC) were studied. The mean AgNOR count per nucleus increased from NOM to ED to OSCC. Tissue showing ED in oral leukoplakia and OSCC cases showed higher counts, wider scatter, and smaller size of AgNOR dots in the nuclei. The study s...