Freeze-drying is often used as a final stage to produce three-dimensional porous matrices for medicine. Because pure solvent crystallizes first during freezing, it acts pore-forming agent. The size of the crystals primarily depends on cooling rate and composition material be frozen. Ultrasonic treatment also affects can control structure matrix. This article describes effect ultrasound (40 kHz,...

Hydrotalcite-type materials, or layered double hydroxide (LDH), are very promising in applying slow-release drug systems through ion exchange and delamination characteristics at acidic pH. In this paper, Zn/Al LDHs 2:1 3:1 molar ratios intercalated with the ibuprofen (IBU) were added to chitosan/alginate membranes. release tests, an increase solubility of membranes could be observed, percentage...

Murine C3H10T1/2 cells were used as a model of mesenchymal stem cells (MSCs) in this study to investigate their capability of proliferation and differentiation in the miniaturized core of small (~100 μm) alginate-chitosan-alginate (ACA) microcapsules. The results show that the cells can survive and proliferate well in the microcapsules, particularly those with a liquid core. Moreover, successfu...

Besides viability protection, a sufficiently prolonged gastrointestinal retention of probiotics has emerged as critically important in improving the functional effectiveness delivery these microorganisms. In this work, we formulated pure, resistant starch-reinforced and chitosan-coated alginate microparticles using an electrospray technique evaluated their performance mucoadhesive probiotic for...

Polyelectrolyte complex (PEC) nanoparticles for delivering model protein drug ovalbumin were prepared from two polysaccharide polymers, alginate and chitosan. The parameters influencing the complex formation were characterised using colloid titration in combination with dynamic light scattering. The polyelectrolyte interactions and morphology of the formed complexes were verified by differentia...

Glucan particles (GPs) are 2–4 m spherical, hollow, porous shells extracted from Baker’s yeast, Saccharomyces cerevisae. The surface of the GPs is composed primarily of 1,3--glucan and the particles are efficiently phagocytosed via receptor-mediated cell uptake by macrophages, phagocytic cells expressing glucan receptors. The hollow cavity of the GPs allows for efficient absorption and encaps...

The aim of the present work was to test the ability of two non-diabetogenic carbohydrates to intranasally deliver the insulinotropic drug repaglinide (REP) for controlling blood glucose level. REP was loaded onto chitosan/alginate nanocomplexes (NCs) suitable for mucosal delivery and uptake. Improved stability and delivery characteristics were obtained by spray drying the selected NCs, yielding...

Better prediction of in vivo drug efficacy using in vitro models should greatly improve in vivo success. Here we utilize 3D highly porous chitosan-alginate complex scaffolds to probe how various components of the glioblastoma microenvironment including extracellular matrix and stromal cells affect tumor cell stem-like state.

The scope of the present work was to establish the role of the polymer charge on its ability to enhance the permeability of the urinary bladder wall. Cationic poly-L-arginine and chitosan, anionic sodium carboxymethyl cellulose (NaCMC) and alginate as well as nonionic hydroxypropyl cellulose (HPC) and hydroxypropyl methylcellulose (HPMC) were tested. The polymers were tested in concentrations f...

Many recent advances in bioprocessing have been enabled by developments in miniaturization and microfluidics. A continuing challenge, however, is integrating multiple unit operations that require distinct spatial boundaries, especially with included labile biological components. We have suggested "biofabrication" as a means for organizing cells and biomolecules in complex configurations while p...