The occurrence of both acid and alkaline ribonuclease activities in liver and kidney was reported in 1954 by Roth (1) and by de Lamirande et al. (2). Since then, investigators have used various methods to purify the intracellular ribonuclease activities (3-8). In our studies, comparatively mild procedures were used for the nuclease preparations (5)) with the hope that the nuclease activities as...

This paper reported the purification and kinetic parameters characterization of an alkaline protease produced from Bacillus subtilis through submerged fermentation process using rice husk as growth supporting substrate (by-product of rice industry) collected from Pearl Rice Mill Hafiz Abad, Faisalabad. Bacillus subtilis was cultured in fermentation medium under some pre-optimized growth conditi...

The purification of the alkaline phosphatases of cow's milk and calf intestinal mucosa as previously described (Morton, 1950, 1953a, 1954a) was initiated in order to enable investigation of the properties of these enzymes. The need for such a study has been well recognized (see Moog, 1946, for example), but hitherto all work has been carried out with crude or partially purified enzyme preparati...

on the response of bone alkaline phosphatase to parathyrin. The results of a typical experiment showing percentage change in alkaline phosphatase activity compared with the hormone-free control are presented in Fig. 2(b). Tissue from 1-day-old animals was unresponsive to parathyrin, that from the 3-day-old animal showed decreased alkaline phosphatase activity and those from 5and 6-day-old anima...