Prevotella spp. are believed to play a central role in ruminal nitrogen metabolism, but little is understood about the genetics and biochemistry of nitrogen assimilation and regulation in these bacteria. The gene encoding a family III glutamine synthetase (GSIII, glnN) in Prevotella bryantii B(1)4 was cloned by Escherichia coli mutant complementation, and enzyme assays as well as Northern blot ...

Ammonium assimilation is catalyzed by two enzymatic pathways, i.e., glutamine synthetase/glutamate synthase (GS/GOGAT) and alanine dehydrogenase (AlaDH) in Amycolatopsis mediterranei U32. Under nitrogen-rich conditions, the AlaDH pathway is the major route for ammonium assimilation, while the GS/GOGAT pathway takes over when the extracellular nitrogen supply is limited. The global nitrogen regu...

in order to investigate the effect of different ratios of ammonium: nitrate on the photosynthesis and fatty acid composition in canola (brassica napus l.) under different levels of salinity, an experiment was conducted as a factorial based on complete randomize design at agricultural research center in east azerbaijan in 2010. the first factor was different ratios of nitrogen sources to ammoniu...

A method has been developed for the production of (13)N-labeled ammonia in usable quantities with negligible contamination. A system was developed and a process for the production of nitrogen-13 ammonium ions from a target material in the form of a dilute solution of ethanol in natural water, i.e. the bombardment of oxygen-16 with protons within the target material. The system includes a device...

A comparative (15)N-tracer study of nitrogen dynamics in headwater streams from biomes throughout North America demonstrates that streams exert control over nutrient exports to rivers, lakes, and estuaries. The most rapid uptake and transformation of inorganic nitrogen occurred in the smallest streams. Ammonium entering these streams was removed from the water within a few tens to hundreds of m...

Microalgae are emerging as the sustainable key source of green fuels and neutraceuticals. In the current study, a strain of Chlorella pyrenoidosa was isolated, identified and biochemically analyzed. Studies were undertaken to enhance its biomass and lipid productivities for sustainable biodiesel production. Growth was monitored in BG11, BBM, Bristol and Fogg’s medium as cell count, O.D, PCV and...

The habitats of fungal pathogens range from environmental to commensal, and the nutrient content of these different niches varies considerably. Upon infection of humans, nutrient availability changes significantly depending on the site and pathophysiology of infection. Nonetheless, a common feature enabling successful establishment in these niches is the ability to metabolise available nutrient...

The aim of this study was to analyse the influence of low-temperature wine fermentation on nitrogen consumption and nitrogen regulation. Synthetic grape must was fermented at 25 and 13 C. Lowtemperature decreased both the fermentation and the growth rates. Yeast cells growing at low-temperature consumed less nitrogen than at 25 C. Specifically, cells at 13 C consumed less ammonium and glutamine...

Wild-type and saccharopine dehydrogenaseless mutant strains of Rhodotorula glutinis grew in minimal medium containing lysine as the sole nitrogen source and simultaneously accumulated, in the culture supernatant, large amounts of a product identified as alpha-aminoadipic-delta-semialdehyde. The saccharopine dehydrogenase and pipecolic acid oxidase levels remained unchanged in wild-type cells gr...

nitrogen is one of the most important and essential lelments for plant growth and development and it has an important role on quantitative and qualitative yield production in agriculture. this study was carried out to evaluate the effects of foliar application of nitrogen on qualitative traits of tomato fruits using a completely randomized design with five treatments and four replications under...