Submit Manuscript | http://medcraveonline.com Abbreviations: AM: Amniotic Membrane; MO: Moringa Oleifera; ITBBR: Institute of Tissue Banking and Biomaterial Research; VEGF: Vascular Endothelial Growth Factor; HGF: Hepatocyte Growth Factor; TGF: Transforming Growth Factor; HLA: Human Leukocyte Antigen; DR: Differential Regulation; MSC: Mesenchymal Stem Cells; SLPI: Secretary Leukocyte Proteinase...

stem cells, the last of which can be obtained from fetal tissues, including amniotic fluid [5]. Analyses of human amniotic fluid have been used as safe and reliable screening tools for fetal genetic and congenital diseases for many years [6]. Amniotic fluid contains cells originating from both embryonic and extraembryonic tissues (amnion, placenta, fetus skin, and respiratory, gastrointestinal,...

We developed a new method for an objective assessment of the meconium content in amniotic fluid. By establishing a standard scale through a serial dilution of a known amount of meconium into the amniotic fluid, we developed a new method "mecometer" that can objectively measure the meconium content in meconium-stained amniotic fluid samples. The objectivity and reliability of this mecometer were...

background :   keratitis caused by pseudomonas  aeruginosa   is often resulted in  severe  corneal  ulcers  and  perforation,  which  leads  to  losses  of  vision. human amniotic membrane (ham) forms the inner wall of the membranous sac which surrounds and protects the embryo during gestation. the purpose of this  study  was  to  evaluate  the  effectiveness  of  the  amniotic  membrane's heal...

Objective: To evaluate amniotic fluid glucose, matrix metalloproteinase (MMP)-9, interleukin (IL)-6, and IL-12 for diagnosing subclinical chorioamnionitis in women with preterm labor. Methods: Forty-four women in preterm labor at 22–35 weeks gestation with suspected subclinical chorioamnionitis underwent amniocentesis. Amniotic fluid analysis included Gram stain, culture, and determination of g...

Six fetal sheep were operated on at 118 to 121 days of gestation. The pulmonary end of the trachea was connected to the gastric end of the esophagus with a section of tubing. This left urine as the only source of amniotic fluid and intramembranous absorption as sole exit. Multiple indwelling fetal vascular, intra-amniotic, allantoic, and a fetal bladder catheter were placed. Beginning 5 days af...

We examined whether the 33-kDa serine protease prostate-specific antigen (PSA) is present in amniotic fluid and, if so, whether its concentration changes with gestational age. Analyzing 115 amniotic fluids with a highly sensitive immunofluorometric procedure, we found PSA in all the amniotic fluids examined and established that its concentration increases with increasing gestational age from 11...

 Amniotic membrane has been employed in the treatment of wounds for almost 100 years, beginning with early application of natural amniotic membrane obtained from labor and delivery to various types of burns and wounds. Amniotic membrane is rich in collagen and various growth factors that support the healing process to both improve wound closure and reduce scar formation. Unique properties...

We present an ultrastructural study of the utilization of human amniotic membrane in the treatment of congenital absence of the vagina in 10 patients. All patients were surgically treated with application of an amniotic membrane graft using the modified McIndoe and Bannister technique. Sixty days after surgery, samples of the vaginal neoepithelium were collected for transmission electron micros...

We describe an enzymatic method for measuring phosphatidylglycerol (PG) at concentrations as low as 0.2 mumol/L in amniotic fluid. Amniotic fluid (1.5 mL) is centrifuged at 10,000g for 20 min to obtain a lamellar body pellet, which is reconstituted with 0.5 mL of buffer. The PG is measured by a two-step enzymatic scheme. Recovery studies demonstrated that the pellet contains > 97% of the PG pre...