A system for detection of trace amounts of protein was developed. Two different monoclonal antibodies against human chorionic gonadotropin (hCG) were covalently bound to latex particles. When the latex particles were mixed with a sample containing hCG, a latex-protein-latex complex (immunocomplex) was formed. The complex was separated from the single latex particles using capillary electrophore...

The quantitative detection of oligomeric nucleic acids including short double-stranded RNA in cells and tissues becomes increasingly important. Here, we describe a method for the detection of siRNA in extracts prepared from mammalian cells, which is based on liquid hybridization with a 32P-labelled probe followed by a nuclease protection step. The limit of detection of absolute amounts of siRNA...

A new time-resolved fluorescence immunoassay system involving use of the europium chelate of 4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid as label is reviewed. This stable chelate by itself is not very fluorescent but, used in multiple labeling strategies, improves the achievable detection limits. By using multiple labeling, streptavidin tailing, and Eu3+ activation, one...

Cyclic voltammetry shows that yeast iso-1-cytochrome c (YCC), chemisorbed on a bare gold electrode via Cys102, exhibits fast, reversible interfacial electron transfer (k(0) = 1.8 x 10(3) s(-1)) and retains its native functionality. Vectorially immobilized YCC relays electrons to yeast cytochrome c peroxidase, and to both cytochrome cd(1) nitrite reductase (NIR) and nitric oxide reductase from P...

by Prof. Larry Davis An item hot off the press is that the Department’s undergraduate programming team, coached by Amol Deshpande, went to Stockholm to compete with squads from all over the world in a programming contest known as the “Battle of the Brains.” Our team placed 20th, and only three other U.S. universities did better or as well – MIT placed 7’th, CMU 12th and Stanford tied with us fo...

Amol Upadhye, Juliana Kwan, Adrian Pope, Katrin Heitmann, 4, 5 Salman Habib, 4, 5 Hal Finkel, and Nicholas Frontiere 7 Department of Physics, University of Wisconsin–Madison, 1150 University Avenue, Madison, WI 53706 Department of Physics and Astronomy, University of Pennsylvania, Philadelphia, PA 19104 High Energy Physics Division, Argonne National Laboratory, 9700 S. Cass Ave., Lemont, IL 604...

seroepidemiological investigation of hepatitis b,c and hiv virus in safe blood donors of babol blood transfusion center   aghajanipoor k.1,2 (md), zandieh t.1(phd)      1 iranian blood transfusion organization-research center   2 amol regional blood transfusion center

considering numerous problems related to the old textures, sustainable development canbe mentioned as the most efficient approach to avoid consequent shortcomings. the methodology adoptedin the present paper is of analytical–descriptive type. using the mentioned method, the data werecollected through the field and documentary studies. the statistical society of present paper includesspecialists...

In order to determine the factors of decay and sourness fig fruits, in summer 2016-17, 60 leaf, fruit stem samples from different regions Tehran, Varamin (Qal'e No), Mazandaran (Amol, Noor Sari), Lorestan Iran a branch sample Italy were collected. We obtained 30 isolates sample. The pathogenicity confirmed by artificially inoculation using fruits. They also characterized based on key ph...