The performance of a new graphite castor oil polyurethane composite electrode is evaluated as an amperometric flow injection detector using hydroquinone (HQ) as a probe. HQ was determined in photographic developers with a minimum detectable net concentration of 100 μmol L-1, and 81 determinations h-1, after optimization of parameters such as the detection potential, sample loop volume, and carr...

The present study was aimed at developing a portable miniaturized FIA system with a two-electrode based amperometric detector for on-site monitoring of residual chlorine in environmental samples. The electrode potential control and current data collection were conducted with a home-designed potentiostat with a size of 150 x 100 x 40 mm and the weight of 350 g. It was confirmed that the amperome...

Fabrication and characterization of a new amperometric chemosensor for accurate formaldehyde analysis based on platinized gold electrodes is described. The platinization process was performed electrochemically on the surface of 4 mm gold planar electrodes by both electrolysis and cyclic voltamperometry. The produced electrodes were characterized using scanning electron microscopy and X-ray spec...

An amperometric enzyme-based sensor-system for the direct detection of formaldehyde in air is under investigation. The biosensor is based on a native bacterial NAD(+)- and glutathione-independent formaldehyde dehydrogenase as biorecognition element. The enzyme was isolated from Hyphomicrobium zavarzinii strain ZV 580, grown on methylamine hydrochloride in a fed-batch process. The sensor depends...

We report a novel and sensitive amperometric sensor for chlorpromazine (CPZ) based on reduced graphene oxide (RGO) and polydopamine (PDA) composite modified glassy carbon electrode. The RGO@PDA composite was prepared by electrochemical reduction of graphene oxide (GO) with PDA. The RGO@PDA composite modified electrode shows an excellent electro-oxidation behavior to CPZ when compared with other...

OBJECTIVES A method is described for construction of an amperometric triglyceride (TG) biosensor using PVA membrane bound enzymes. DESIGN AND METHODS A mixture of commercial lipase, glycerol kinase, glycerol-3-phosphate oxidase and horseradish peroxidase was co-immobilized onto polyvinyl alcohol (PVA) membrane through glutaraldehyde coupling. The biosensor measures current when polarized at 0...

An amperometric horseradish peroxidase (HRP) biosensor based on multilayer films containing carbon nanotubes (CNTs) and HRP was developed. With the pH regulation of the dispersion solution of CNTs, the sensitivity of the HRP multilayer film biosensor is tunable by the control of the dissociation of CNTs. The successful formation of multilayers was confirmed by UV-visible spectroscopy. The featu...

The determination of creatinine levels in biological fluids is an increasingly important clinical requirement. Amperometric biosensors have been developed based on a three-enzyme system which converts creatinine to amperometrically measurable hydrogen peroxide. The development of the amperometric creatinine biosensor has been slow due the complexity of the three-enzyme system. This paper, the f...

The potential capabilities and analytical performance of lab-on-valve (LOV) manifold as a front end to amperometry have been explored for the on-line determination of morin. Meanwhile, the electrochemical behaviors of morin were investigated based on polyvinylpyrrolidone- (PVP-) doped carbon paste electrode (CPE), which found that PVP can significantly improve its oxidation peak current. The ex...