In his original study of Pseudomonas saccharophila, Doudoroff (1940) found that an inducible extracellular amylase was formed when cells were grown with soluble starch or maltose. He also observed indications of intracellular amylase activity. The present study was undertaken in order to characterize further the amylase(s) of P. saccharophila. Two enzymes with different physical and enzymatic p...

For most multidomain proteins the thermal unfolding transitions are accompanied by an irreversible step, often related to aggregation at elevated temperatures.As a consequence the analysis of thermostabilities in terms of equilibrium thermodynamics is not applicable, at least not if the irreversible process is fast with respect the structural unfolding transition. In a comparative studywe inves...

Occurrence of chalkiness is a major problem as it is an important parameter in determining the quality of rice grains. High temperature during the grain filling period results in loose packing starch granules that could be due to the involvement of starch degrading enzymes i.e., amylases. Therefore, two rice genotypes viz., PAU 3699-13-2-1-1 and PR122 were sown at two different dates. Thirty-da...

The aim of this study was screening fungi strains, isolated from manipueira (a liquid subproduct obtained from the flour production of Manihot esculenta), for amylases production and investigating production of these enzymes by the strain Aspergillus 6V4. The fungi isolated from manipueira belonged to Ascomycota phylum. The strain Aspergillus 6V4 was the best amylase producer in the screening a...

Crystal structures of human pancreatic alpha-amylase (HPA) in complex with naturally occurring inhibitors have been solved. The tetrasaccharide acarbose and a pseudo-pentasaccharide of the trestatin family produced identical continuous electron densities corresponding to a pentasaccharide species, spanning the -3 to +2 subsites of the enzyme, presumably resulting from transglycosylation. Bindin...

The alpha-amylases in the salivary glands of Lygus hesperus Knight and L. lineolaris (Palisot de Beauvois) were isolated and purified by ion exchange chromatography, and by isoelectric focusing, respectively. The alpha-amylase from L. hesperus had an isoelectric point (pI) of 6.25, and a pH optimum of 6.5. The specific activity of alpha-amylases in the salivary glands of L. hesperus was 1.2 U/m...