BACKGROUND Ro/SS-A autoantibodies associated with systemic lupus erythematosus (SLE) and Sjögren syndrome may be missed during routine screening for antinuclear autoantibodies (ANA) by immunofluorescence using HEp-2 cells. AIMS To investigate the use of HEp-2 cells transfected with human 60 kDa Ro/SS-A for routine detection of these antibodies. METHODS 10,500 sera were screened at a dilutio...

Correspondence: Silvina Gutiérrez Centro de Microscopía Electrónica. Facultad de Ciencias Médicas. Universidad Nacional de Córdoba. Córdoba. Argentina. [email protected] [email protected] The classification of lupus nephritis was revised by the ISN/RPS in 2003. The increasingly recognized phenomenon of apparent minimal change disease (MCD) in the context of systemic lupus erythemato...

<p class="abstract">Lupus panniculitis of scalp is an uncommon manifestation lupus erythematosus, which presents as non-scarring linear, arched, or annular alopecia along the lines Blaschko. A 7 year old child presented with localised linear on scalp. Examination revealed a single alopecic patch left parietal There were no associated systemic complaints. Skin biopsy showed atrophic lining...

There are as many as one-third of the systemic lupus erythematosus (SLE) patients who suffer from dry eye syndrome. To this date, dry eye syndrome in SLE patients is believed to be caused by secondary Sjogren syndrome (sSS). However, there is increasing evidence for possible independency of dry eye syndrome and sSS in patients suffering from autoimmune diseases. The purpose of this retrospectiv...

INTRODUCTION Autoantibodies to the ribosomal P proteins represent a highly specific marker for the diagnosis of systemic lupus erythematosus, where they have been associated with certain clinical manifestations. Historically, autoantibodies against ribosomal P proteins have been detected by indirect immunofluorescence, immunodiffusion, immunoblot, and other immunoassays. More recently, enzyme-l...

We present a retrospective review of DNA immunoadsorption (DNA-IA) therapy on clinical symptoms as well as indicators in pediatric cases with systemic lupus erythematosus (SLE), and follow up the shortterm curative effects. 16 SLE cases were treated by DNA-IA for 3 times every other day. We observed the changes on clinical manifestations and immunological indicators, in order to compare the alt...

Anti-nuclear antibody (ANA) testing is widely used as a screening test in Autoimmune diseases. The most commonly used method for ANA testing is ANAimmunofluorescence assay (ANA-IFA). This study compared the diagnostic performance of Enzyme linked immuno sorbent assay (ELISA) with IFA for the detection of ANA. 125 samples were tested which included 75 samples from patients with clinical suspicio...

The following guideline presents a series of recommendations based on published medical literature for use of the antinuclear antibody (ANA) test and tests for specific autoantibodies to nuclear antigens in the diagnostic evaluation, prognostic assessment, and monitoring of patients with systemic rheumatic diseases. The guideline emphasizes the need for clinical evaluation to improve the useful...

BACKGROUND Antinuclear antibodies (ANA) and anti-double stranded DNA (anti-dsDNA) are often tested as a screening tool in patients with suspected systemic lupus erythematosus or connective tissue diseases. ANA can be seen in healthy controls (HC) and patients with multiple medical problems (MMP). OBJECTIVE To determine the sensitivity and specificity of ANA and anti-dsDNA in SLE patients, usi...

Systemic lupus erythematosus (SLE) is characterized by high-avidity IgG antinuclear antibodies (ANAs) that are almost certainly products of T cell-dependent immune responses. Whether critical amino acids in the third complementarity-determining region (CDR3) of the ANA originate from V(D)J recombination or somatic hypermutation (SHM) is not known. We studied a mouse model of SLE in which all so...