Disulfide bonds are important for the correct folding, structural integrity, and activity of many biotechnologically relevant proteins. For synthesis and subsequent secretion of these proteins in bacteria, such as the well-known "cell factory" Bacillus subtilis, it is often the correct formation of disulfide bonds that is the greatest bottleneck. Degradation of inefficiently or incorrectly oxid...

Allelic replacement in staphylococci is frequently aided by antibiotic resistance markers that replace the gene(s) of interest. In multiply modified strains, the number of mutated genes usually correlates with the number of selection markers in the strain's chromosome. Site-specific recombination systems are capable of eliminating such markers, if they are flanked by recombinase recognition sit...

The sensor kinase NreB from Staphylococcus carnosus contains an O(2)-sensitive [4Fe-4S](2+) cluster which is converted by O(2) to a [2Fe-2S](2+) cluster, followed by complete degradation and formation of Fe-S-less apo-NreB. NreB.[2Fe-2S](2+) and apoNreB are devoid of kinase activity. NreB contains four Cys residues which ligate the Fe-S clusters. The accessibility of the Cys residues to alkylat...

Characterization of a nitrite reductase-negative Staphylococcus carnosus Tn917 mutant led to the identification of the nir operon, which encodes NirBD, the dissimilatory NADH-dependent nitrite reductase; SirA, the putative oxidase and chelatase, and SirB, the uroporphyrinogen III methylase, both of which are necessary for biosynthesis of the siroheme prosthetic group; and NirR, which revealed n...

The aim of the research was to estimate possibility using mixed bacteria cultures consisting Lactobacillus fermentum S8 and Staphylococcus carnosus ATCC 51365 in meat curing process with a reduced amount sodium nitrite study effect on residual nitrites nitrates, nitrosyl pigments content, colour, pH, redox potential, microbiologic, sensory quality cooked product. performed heat treated three-mo...