background: in lupus nephritis (ln), deposition of pathogenic autoantibodies in the glomeruli is mediated via cross-reactivity with alpha-actinin. association of serum alpha-actinin antibody (aαa) with ln has been shown in a few studies but the results are controversial. methods: eighty patients into entered the study. the diagnosis of sle was confirmed according to the american college of rheu...

background: immunopathological and inflammatory processes play important roles in the initiation and development of ischemic heart disease (ihd). objective: the aim of this study was to evaluate the serum levels of several autoantibodies including rheumatoid factor (rf), anti-nuclear antibodies (ana), anti-small nuclear ribonucleoprotein (anti-sm), anti-phosphatidylserine (anti-ps) and anti-car...

background: auto antibodies to zona-pellucida (aza) seem to be important autoantibodies implicated in reproduction, with substantial role in both endocrine and reproductive functions of the human ovary. there are some debates on the relation of aza with infertility, repeated in vitro fertilization (ivf) attempts, and outcome of it. objective: in this study, we assessed the presence of aza in th...

QuantiFERON®-TB Gold in-tube (QFT-GIT) supernatants may be important samples for use in assessment of anti-tuberculosis (TB) antibodies when only limited volumes of blood can be collected and when a combination of antibody and cytokine measurements are required. These analytes, when used together, may also have the potential to differentiate active pulmonary TB (APTB) from latent TB infection (...

background: cholera, an infectious disease caused by vibrio cholerae , is primarily transmitted by ingestion of contaminated food or water. in severe cases, cholera may lead to severe dehydration, metabolic acidosis, and ultimately, hypovolemic shock and death.   methods: in this study v.cholerae non-o1 was cultured in suitable media. lps was extracted from the surface of  bacteria by hot pheno...

Objective(s): Blocking of vascular endothelial growth factor (VEGF) plays a pivotal role in inhibition of metastasis and is a target for development of anti-angiogenic agents. In this study, a peptide-based vaccine was designed and its potential for induction of humoral immune responses, generation of neutralizing antibodies, inhibition of tumor growth and metastasis w...

Background: Invariant natural killer cells (iNKT) are an important immunoregulatory T cell subset. Currently several flow cytometry-based approaches exist for the identifi-cation of iNKT cells, which rely on using the 6B11 monoclonal antibody or a combina-tion of anti-Vα24 and anti-Vβ11 antibodies. Objective: The aim of this study was to compare the ability of two flow cytometry-based methods f...

objectives: clinical laboratory health care workers can become infected through their occupation with blood-borne pathogens by percutanous injuries and mucocutaneous blood contacts such as cuts, needle sticks, splashes to mucous membranes or other body injuries. the purpose of this study was to determine the seroprevalence of, hepatitis c virus (hcv), and some of the risk factors in medical lab...