Diagnosis of larval migrans (LM) is usually done by immunodiagnostic methods. These methods, however, simply show the presence or absence of antibody but not the active infection of the patients. Therefore, we aimed to establish a diagnostic method for detecting circulating Toxocara canis antigen using a sandwich, ELISA. Monoclonal antibodies (MAb) were produced against the excretory-secretory ...

Antigen-antibody interactions are a fundamental subset of protein-protein responsible for the “survival fittest.” Determining interacting interface antigen, called an epitope, and that on antibody, paratope, is crucial to antibody development. Because each antigen presents multiple epitopes (unique footprints), sophisticated approaches required determine target region given antibody. Although X...

Conventional serum antibody titer, which expresses level, does not provide antigen binding avidity of the variable region antibody, is essential for defense response to infection. Here, we quantified anti-SARS-CoV-2 receptor-binding domain (RBD) by competitive binding-inhibition activity (IC50) between SARS-CoV-2 S1 immobilized on DCP microarray and various RBD doses added expressed as 1/IC50 n...

This study examines the immune responses against some stress proteins of Mycobacterium leprae in leprosy patients with and without leprosy reactions. Leprosy patients showed a higher level of antibodies to all antigens compared to healthy controls. The antibody response to 18kDa antigen was significantly higher in patients with Type 1 reaction compared to those of TT or borderline patients with...

Further evidence implicating murine leukemia-like virus in the disorders of NZB mice was afforded by a study of antigens associated with murine leukemia virus (MuLV). MuLV group antigens were prevalent in extracts of spleen, kidney, and, to a lesser extent, thymus throughout a substantial portion of the life span of NZB mice as well as in extracts of lymphomas and sarcomas indigenous to the str...

A novel lateral flow immunoassay biosensor, combining the receptor-ligand reaction and antigen-antibody was developed for detection of ?-lactam antibiotics in milk. The receptor protein, carboxy-terminal domain sensor-transducer mutant from Bacillus. licheniformis, served as recognition element. Colloidal gold (CG)-labelled antibody-receptor ternary complex applied a signal transducer probe. pr...

The reactions of lysozyme with the specific monoclonal antibody D1.3, its Fv fragment and a mutant of the Fv, were studied under conditions of reduced water activity through the addition of the cosolutes glycerol, ethanol, dioxane and methanol. Titration calorimetry, BIAcoreTM and ultracentrifugal analyses were used to determine enthalpy of reactions and affinity constants. There was a decrease...

The diagnosis of congenital cytomegalovirus (CMV) infection is often accomplished by the detection of circulating antibody directed against CMV. We devised a method for measuring CMV-specific immunoglobulin M (IgM) based on the isolation of IgM antibody by reaction with a solid phase coated with antihuman IgM. The determination of IgM antibody specific for CMV was accomplished by the subsequent...

The gonococcal complement-fixation test (GCFT), based on the same principle as the Wassermann reaction (WR), has been in use for over 60 years. The test was applied for the first time, using a culture of the gonococcus as antigen, by Muller and Oppenheim (1906) to establish the diagnosis in a case of arthritis. Teague and Torrey (1907) suggested that negative results in the GCFT were due to dif...

The means by which interaction of antigen and antibody may lead to disease is not well understood. Arthus (1) observed that repeated subcutaneous injections of horse serum in rabbits elicited progressively severe local inflammatory reaction. From clinical observations on serum disease in man yon Pirquet (2) suggested "that a disease might be due indirectly to an antibody" and he coined the term...