the attacks of convulsion or death of the mouse and rat caused by injecting the pyrimidine moiety of thi amine, i. e. 2-methyl-6amino-5-hydroxymethylpyrimidine, (conveniently call ed "toxopyrimidine"), were protected by the substance, contained in the rice-bran, and they confirmed that vitamin B6-complex was the typical antimetabolite against toxopyrimidine be cause of partial similarity of the...

Azathioprine (Imuran) was administered to seven patients with ulcerative colitis suffering from a relapse which could not be controlled by adrenocortical steroids. In four patients remission started one to two weeks after initiation of antimetabolite therapy. Corticosteroid administration was continued concurrently with azathioprine, but the dosage could be reduced and in one case they were wit...

The outcome of acute myelogenous leukemia (AML) therapy depends on the propensity of leukemic blasts to accumulate ara-CTP, the active triphosphate of cytarabine (ara-C). We identified sterile α motif and HD domain-containing protein 1 (SAMHD1) as an ara-CTPase that protects cancer cells from cytarabine-induced toxicity. Therefore, we propose targeting SAMHD1 as a strategy to potentiate cytarab...

We developed a negative counterselection system for Pseudomonas putida based on the uracil phosphoribosyl-transferase (UPRTase) and the sensitivity against the antimetabolite 5-fluorouracil (5-FU). We constructed a P. putida strain that is resistant to 5-FU and constructed vectors for the deletion of the surface adhesion protein gene, the flagellum biosynthesis operon and two endonuclease genes...

comorbidities, many patients eventually relapse or do not qualify for standard therapies 1,2. Additional treatment options that improve tolerability while maximizing efficacy are therefore needed in those settings. Bendamustine hydrochloride is a bifunctional alkylating agent with clinical activity across a number of cancers, including breast cancer, small-cell lung cancer, multiple myeloma, cl...

Methotrexate is known as a folate antimetabolite. The side effects of methotrexate are not usually seen in low dosages. Here we present patient with inflammatory colitis due to use for psoriasis.A 29 year-old male who was diagnosed psoriasis 1 year ago and started 2 weeks; applied emergency acute abdomen symptoms. According the tests examinations toxicity. observed abdominal pain without surgic...