We have studied the in vitro inactivation/dissociation and subsequent reactivation/re-assembly of peroxisomal alcohol oxidases (AO) from the yeasts Hansenula polymorpha and Pichia pastoris. Both proteins are homo-oligomers consisting of eight identical subunits, each containing one FAD as the prosthetic group. They were both rapidly inactivated upon incubation in 80% glycerol, due to their diss...

Adaptive optics (AO) in astronomical imaging is a technique to improve the quality of image by compensating aberrations induced by atmospheric turbulence. Digital holographic AO (DHAO) is one attractive option to implement AO scheme because it is capable of directly measuring the phase profile of aberration without complicated calculation or loss of resolution of CCD. Hence, if applicable, DHAO...

We have created a new autonomous laser-guide-star adaptive-optics (AO) instrument on the 60-inch (1.5-m) telescope at Palomar Observatory called Robo-AO. The instrument enables diffraction-limited resolution observing in the visible and near-infrared with the ability to observe well over one-hundred targets per night due to its fully robotic operation. RoboAO is being used for AO surveys of tar...

Echocardiograms were performed in 20 infants and children with isolated ventricular septal defects (VSD) undergoing cardiac catheterization. The magnitude of the left-to-right shunt was expressed as the pulmonary-to-systemic flow ratio (Qp/Os) and was compared to a ratio of the echographic left atrial (LA) diameter to the aortic root (Ao) diameter (LA/Ao). The Qp/Qs was also compared to LA/m2. ...

We sought to quantify agreement by different assessors of the AO classification for distal fractures of the radius. Thirty radiographs of acute distal radial fractures were evaluated by 36 assessors of varying clinical experience. Our findings suggest that AO 'type' and the presence or absence of articular displacement are measured with high consistency when classification of distal radial frac...

Two-photon fluorescence microscopy provides a powerful tool for deep tissue imaging. However, optical aberrations from illumination beam path limit imaging depth and resolution. Adaptive Optics (AO) is found to be useful to compensate for optical aberrations and improve image resolution and contrast from two-photon excitation. We have developed an AO system relying on a MEMS Deformable Mirror (...

Ascorbate oxidase (AO, EC 1.10.3.3) catalyzes the oxidation of ascorbate (AsA) to yield water. AO over-expressing plants are prone to ozone and salt stresses, whereas lower expression apparently confers resistance to unfavorable environmental conditions. Previous studies have suggested a role for AO as a regulator of oxygen content in photosynthetic tissues. For the first time we show here that...

in this article the relationship between the amount of seasonal rainfall (autumn, winter and spring) and climate indices in iran have been studied. the climate indices (the southern oscillation(soi),the north atlantic oscillation(nao), arctic oscillation(ao),nino 3/4,the multivariate enso index(mei),trans_ nino index(tni) and the pacific decadal oscillation(pdo) are used as independent variable...

Adaptive Optics (AO) systems are widely used in many scientific and medical applications, such as astronomy, laser systems and microscopes, in order to improve the resolution of the image by actively sensing and compensating the optical aberration in the system. This paper aims at improving the performance of a closed-loop AO system with Piezo-driven Deformable Mirror (PDM) and high-sampling-ra...

The Giant Magellan Telescope (GMT) includes adaptive optics (AO) as an integral component of its design. Planned scientific applications of AO span an enormous parameter space: wavelengths from 1 to 25 μm, fields of view from 1 arcsec to 8 arcmin, and contrast ratio as high as 10. The integrated systems are designed about common core elements. The telescope’s Gregorian adaptive secondary mirror...