the aim of this study was the increasing of leachate quality using integrated membrane bioreactor (mbr). the reactor was fed with treated leachate with overall 70-1360 mg/l chemical oxygen demand (cod). the analysis of cod, biochemical oxygen demand (bod5), total suspended solids (tss), and total dissolved solids (tds) were performed in feed and filtrate, whenever the system reached steady stat...

An improved method for the colorimetric determination of urea is described. The method is simple, requiring a deproteinized filtrate obtained after treatment of a small quantity of whole oxalated blood, serum, plasma or urine with 12.5% trichloracetic acid containing 50 g. activated carbon. The addition of Ehrlich's reagent to this filtrate results in the immediate production of a stable yellow...

A gene (bgl) encoding a β-glucosidase in thermophilic actinomycete Thermobifida fusca NTU 22 was cloned into a Yarrowia lipolytica expression system. Heterologous expression resulted in extracellular β-glucosidase production with activity as high as 630 U/mL in a Hinton flask culture filtrate. This recombinant β-glucosidase was purified 9.2-fold from crude culture filtrate by DEAE-Sepharose FF ...

The dimorphic fungal pathogen Histoplasma capsulatum causes respiratory and systemic disease. Within the mammalian host, pathogenic Histoplasma yeast infect, replicate within, and ultimately kill host phagocytes. Surprisingly, few factors have been identified that contribute to Histoplasma virulence. To address this deficiency, we have defined the constituents of the extracellular proteome usin...

Most applications need to invoke some Application Programming Interfaces (APIs), e.g. JDK (Java Development Kit) API. When invoking those APIs, applications must follow some API constraints. Violation of these constraints will lead to some severe program defects. To detect this kind of defects, lots of static and dynamic approaches are explored, using formally described API constraints. While m...

B ECAUSE of the pressing need for conserving both the patient’s blood and laboratory time, an attempt has been made to modify the Karr urea method (1) for semimicro amounts of Somogyi filtrate (2). Since this serum filtrate was already being used for glucose in our clinical laboratory, the saving of time and material which such a method would bring-if accurate enough for clinical purposes-was o...

Crude extracts of filtrates of cultures of Fusarium sambucinum NRRL 13495 were acetylated or hydrolyzed. After chromatography on cartridge columns of silica gel and recrystallization three times from mixtures of ethyl acetate and hexane, 3,4,15-triacetoxyscirpenol (435 +/- 10 mg/liter of filtrate; mean +/- standard error [n = 3]) and the parent alcohol scirpentriol were isolated (261 +/- 29 mg/...

API-1 (pyrido[2,3-d]pyrimidines) is a novel small-molecule inhibitor of Akt, which acts by binding to Akt and preventing its membrane translocation and has promising preclinical antitumor activity. In this study, we reveal a novel function of API-1 in regulation of cellular FLICE-inhibitory protein (c-FLIP) levels and TRAIL-induced apoptosis, independent of Akt inhibition. API-1 effectively ind...

Malignant mesothelioma (MM) is a tumor arising from mesothelium. MM patients' survival is poor. The polyphenol 4',5,7,-trihydroxyflavone Apigenin (API) is a "multifunctional drug". Several studies have demonstrated API anti-tumoral effects. However, little is known on the in vitro and in vivo anti-tumoral effects of API in MM. Thus, we analyzed the in vitro effects of API on cell proliferation,...