The extraction of xylanase was performed using an aqueous two-phase system (ATPS) based on polyethylene glycol (PEG1540) and various salts. Preliminary studies in a batch extractor showed that the highest efficiency, E = 79.63 ± 5.21%, purification factor, PF 1.26 0.25, were obtained with sodium citrate dihydrate-H2O-PEG1540-based ATPS for time 10 min. process optimized experimental Box-Behnken...

Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), pH (4.0-6.0) and temperature (25-35 °C), obtainin...

Aqueous two-phase systems (ATPSs) composed by UCON (ethylene oxide/propylene oxide copolymer) and potassium phosphate salts were for the first time evaluated in the recovery of Peniophora cinerea laccase from complex fermented medium. The ATPSs were obtained by combining the random copolymer UCON with KH2PO4, potassium phosphate buffer pH 7 or K2HPO4. According to the results, protein partition...

The use of new synthesized ammonium-based ionic liquids was explored as an alternative to the current process implemented in betanin extraction from red beet juice, resulting high yields: 70% and 82%. Betanin is a vegetal pigment that has been applied large variety products food industry, which important, for it can work substitute synthetic dyes used nowadays. Additionally, kosmotropic salt so...

An Aqueous Two-Phase System (ATPS) was employed for the first time for the separation and purification of pectinase from mango (Mangifera Indica Cv. Chokanan) peel. The effects of different parameters such as molecular weight of the polymer (polyethylene glycol, 2,000-10,000), potassium phosphate composition (12-20%, w/w), system pH (6-9), and addition of different concentrations of neutral sal...

Collagenases are proteolytic enzymes capable of degrading both native and denatured collagen, reported to be applied in industrial, medical and biotechnological sectors. Liquid-liquid extraction using aqueous two-phase system (ATPS) is one of the most promising bioseparation techniques, which can substitute difficult solid-liquid separation processes, offering many advantages over conventional ...

Abstract Background Bacteriocins are generally defined as ribosomally synthesized peptides, which produced by lactic acid bacteria (LAB) that affect the growth of related or unrelated microorganisms. Conventionally, extracted bacteriocins purified precipitation, where ammonium sulphate is added to precipitate out protein from solution. Main text To achieve high purity bacteriocins, a combinatio...