We have studied the effect of ascorbic acid on voltage-dependent calcium channels in pancreatic [3 cells. Using the whole-cell and perforated-patch variants of the patch clamp technique to record calcium tail currents, we have shown that the slowly deactivating (SD) calcium channel, which is similar to the T-type channel in other cells, is inhibited in a voltage-dependent manner by ascorbic aci...

Pulmonary function tests (PFTs) routinely implemented in clinics are the first step diagnosis of idiopathic pulmonary fibrosis. Evaluation PFTs mouse model fibrosis accompanied by histological readouts may improve clinical predictability new therapeutic candidates. Forced vital capacity (FVC) is considered most predictive restrictive disorders. This study aimed to test improvement PFT mice lung...

T his symposium is devoted to evidence based medicine (EBM) and the ethical issues it raises. Since Sir Archie Cochrane’s seminal Nuffield Provincial Hospitals Trust lectures in 1972 and their publication as the Rock Carling monograph for that year, Effectiveness and Efficiency: Random Reflections on Health Services, the idea that medical interventions and health services should be evaluated an...

Belleville, R. E., and Fraser, H. F. (1957). J. Pharmacol. exp. Ther., 120, 469. Berger, R. J., and Oswald, I. (1962). 7. ment. Sci., 108, 457. Collier, H. 0. J. (1965). Nature (Lond.), 205, 181. Dement, W. (1960). Science, 131, 1705. and Kleitman, N. (1957). Y. exp. Psychol., 53, 339. Evans, J. I., and Oswald, I. (1965). Electroenceph. clin. Neurophysiol., In press. Fraser, H. F., Isbell, H., ...

Paracrine factors are currently considered to be the major mechanism through which mesenchymal stem cells (MSCs) exert their actions. The aim of this study was to investigate the protective effects of conditioned medium (CM) from bone marrow mesenchymal stem cells (MSC) on bleomycin (BLM)‑induced lung injury and fibrosis, both in vitro and in vivo. A549 human non‑small cell lung cancer epitheli...

The Saccharornyces cerevisiae CDC3, CDClO, CDCl I , and CDC12 genes encode a family of homologous proteins that are not closely related to other known proteins [Haarer BK, Ketcham SR, Ford SK, Ashcroft DJ, and Pringle JR (submitted)]. Temperature-sensitive mutants defective in any of these four genes display essentially identical pleiotropic phenotypes that include abnormal cell-wall deposition...

OBJECTIVE Secretoglobin (SCGB) 3A2 is a novel lung-enriched cytokine, previously shown to exhibit anti-inflammatory, growth factor, and anti-fibrotic activities. The latter activity was demonstrated using exogenously-administered recombinant SCGB3A2 in the bleomycin (BLM)-induced pulmonary fibrosis model. Whether SCGB3A2 exhibits anti-fibrotic activity in vivo is not known. METHODS Mice null ...

Purpose: To evaluate the effects of H6552 extract in inhibiting transforming growth factor (TGF)mediated pulmonary fibrosis in vitro and in vivo. Methods: Maximum-nontoxic dose (MNTD) of Actinomyces H6552 extract was determined using 3(4,5-dimethylthiazol-2-yl)-2,5-diphhenyltetrazolium bromide (MTT) assay. Effect of the extract on IMR90 lung fibroblasts proliferation was determined by calculati...

Background: Pulmonary fibrosis is a chronic and usually untreatable fatal lung disease. It can result from many endogenous or exogenous causes, such as infection, chronic inflammatory diseases, environmental toxins, chemical poisoning, radiation and chemotherapy. The accumulation of collagen, largely type I collagen rich extracellular matrix (ECM) is the hallmark of lung fibrosis. Current thera...