Soybean plants can fix atmospheric N2 in the root nodule, a symbiotic organ with rhizobia. The primary forms of N transported from fixation are ureides, allantoate, and allantoin, supplemented asparagine. nitrate absorbed roots is to shoots NO3− asparagine little portion ureides. concentrations N-metabolites were analyzed by capillary electrophoresis after supplying various urea, precursors all...

When Jensen sarcoma cells were cultured in medium devoid of free L-asparagine, the cell population decreased exponen tially for 10 days. The surviving cells then began an exponen ti@alproliferation phase. The resulting population did not quire asparagine to maintain proliferation. Population growth curves and cloning studies showed that, per 106 original cells, approximately 102 variant cells w...

The biosensor for monitoring asparagine levels in Fruit juices was developed in present research work. Lasparaginase (biocomponent) has been extracted from Withania somnifera (L) Dunal, and immobilized into TEOS hydrosol gel-chitosan matrix. Kinetic characterization of both native and immobilized enzyme was compared. Response time studies had been carried out by Phenol Red indicator coimmobiliz...

(15)NH(4) (+) and [(15)N](amide)-glutamine externally supplied to detached nodules from soybean plants (cv. Tamanishiki) were incorporated within nodule tissues by vacuum infiltration and metabolized to various nitrogen compounds during 60 minutes of incubation time. In the case of (15)NH(4) (+) - feeding, the (15)N abundance ratio was highest in the amide nitrogen of glutamine, followed by glu...

Benzalacetone synthase (BAS), a plant-specific type III polyketide synthase (PKS), catalyzes a one-step decarboxylative condensation of malonyl-CoA and 4-coumaroyl-CoA to produce the diketide benzalacetone. We solved the crystal structures of both the wild-type and chalcone-producing I207L/L208F mutant of Rheum palmatum BAS at 1.8 A resolution. In addition, we solved the crystal structure of th...

background: l-tryptophan is used widespread in the pharmaceutical industry. the majority of l-trp production depends on microbial processes that produce l-tryptophan from indole and l-serine. these processes are very costly due to the costs of precursors, especially l-serine. use of inexpensive substitutions as the l-serine source of l-tryptophan production enables us to reach a cost-effective ...

After the first isolation of asparagine in 1806 by Vauquelin and Robiquet (l), there were numerous studies of its properties and physiological significance (2-4). Piria (5) in 1848 was probably the first to adduce evidence for a physiological role of asparagine during the life cycle of plants, and also to observe its transformation or metabolism by yeast. However, it was not until approximately...

A cDNA clone, designated as PvNAS2, encoding asparagine amidotransferase (asparagine synthetase) was isolated from nodule tissue of common bean (Phaseolus vulgaris cv. Negro Jamapa). Southern blot analysis indicated that asparagine synthetase in bean is encoded by a small gene family. Northern analysis of RNAs from various plant organs demonstrated that PvNAS2 is highly expressed in roots, foll...