Short indicator RNA sequences (<100 bp) persist after autoclaving and are recovered intact by molecular amplification. Primers targeting longer sequences are most likely to produce false positives due to amplification errors easily verified by melting curves analyses. If short indicator RNA sequences are used for virus identification and quantification then post autoclave RNA degradation method...

Henriques and Gjaldbaek,l in an article devoted chiefly to a study of the above subject with the form01 titration, find that, when proteins are hydrolyzed with acid, both amino nitrogen and ammonia increase up to a certain point, at which the amino nitrogen attains its maximum. When hydrolysis is continued beyond this point, a transformation of amino nitrogen into ammonia follows, indicating a ...

OBJECTIVES The purpose of this study was to evaluate the Vickers hardness number (VHN) and the in vitro marginal adaptation of inlay restorations of three hybrid composite resins (Filtek Z250, Opallis and Esthet-X) subjected to two post-cure treatments. MATERIAL AND METHODS For the microhardness test, three different groups were prepared in accordance with the post-cure treatments: control gr...

AIM To compare the cyclic fatigue resistance of HyFlex CM, Twisted Files (TF), K3XF, Race, and K3, and evaluate the effect of autoclave sterilization on the cyclic fatigue resistance of these instruments both before and after the files were cycled. METHODOLOGY Five types of NiTi instruments with similar size 30, .06 taper were selected: HyFlex CM, TF, K3XF, Race and K3. Files were tested in a...

Blankets may be disinfected in steam at subatmospheric pressures by temperatures below boiling point inside a suitably adapted autoclave chamber. The chamber and its contents are thoroughly evacuated of air so as to allow rapid heat penetration, and steam is admitted to a pressure of 10 in. Hg below atmospheric pressure, which corresponds to a temperature of 89 degrees C. Woollen blankets treat...

To know the conditions of contamination by the chemicals in the experimental animal diets, the contents of nitrosodimethylamine (NDMA) were analyzed in the diets for mice and rats. The raw materials of the diets were also analyzed. In addition, NDMA levels were determined in the diets which were stored long or heated in the autoclaves. The averages of NDMA in these diets were less than 10 ppb. ...

Although the European reports highlight an increase in community-acquired Legionnaires' disease cases, the risk of Legionella spp. in private houses is underestimated. In Pisa (Italy) we performed a three-year survey on Legionella presence in 121 buildings with an independent hot water production (IB); 64 buildings with a central hot water production (CB); and 35 buildings with a solar thermal ...

A method has been developed for measuring formaldehyde concentrations in a subatmospheric steam-formaldehyde autoclave. Data obtained using this method indicate that the concentration of formaldehyde in the chamber atmosphere is not homogeneous and that it decreases rapidly with time. The penetration of formaldehyde vapour into narrow tubes has also been investigated and was shown to be depende...