The genomic revolution in oncology will entail mutational analyses of vast numbers of patient-matched tumor and normal tissue samples. This has meant an increased risk of patient sample mix up due to manual handling. Therefore, scalable genotyping and sample identification procedures are essential to pathology biobanks. We have developed an efficient alternative to traditional genotyping method...

A method for fluorescent labeling of PCR products has been developed. This method consists in a two-step procedure in which a first exponential classical PCR is followed by a "linear amplification". This second step relies on incorporation of fluorescent dNTP (dUTP or dCTP) in order to label the product on only one strand. The products can be applied without prior purification directly to a gel...

This paper provides an overview of a new method for approaching beat sequencing. As we have come to know them drum machines provide means to loop rhythmic patterns over a certain interval. Usually with the option to specify different beat divisions. What I developed and propose for consideration is a rethinking of the traditional drum machine confines. The Sinkapater is an untethered beat seque...

<em>Power sequencers</em> are used in audio sound system accessories or PLC factories industries so that and production equipment can run sequentially according to the order of their functions. So later there will be no malfunctions errors result losses cause damage industry. However, power sequencer is for at this time cannot changed - change delay value only turn on off one channe...

In India, Holstein and its crosses are being used extensively in breeding programmes and all these breeding bulls are screened for autosomal recessive genes. Blood samples are collected in ethylenediaminetetraacetic acid (EDTA) coated tubes and DNA was isolated by using phenol-chloroform method. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) wereperformed by using...

This paper introduces a powerful novel sequencer hardware for controlling computational machines and for structured DMA (direct memory access) applications. The paper introduces the principles and the design of a novel class of this sequencer hardware which supports two-dimensional memory address space or at least the two-dimensional visualization of the traditional one-dimensional address spac...

The most efficient receiver-driven multicast congestion control protocols use dynamic channels. This means that each group has a cyclic rate variation with a continuously decreasing phase. Despite promising results in terms of fairness, using efficiently these dynamic groups could be a challenging task for application programmers. This paper presents a sequencer which maps out application data ...

The Neurohedron is a multi-modal interface for a nonlinear sequencer software model, embodied physically in a dodecahedron. The faces of the dodecahedron are both inputs and outputs, allowing the device to visualize the activity of the software model as well as convey input to it. The software model maps MIDI notes to the faces of the device, and defines and controls the behavior of the sequenc...

Pausing of DNA polymerase can indicate the presence of a DNA structure that differs from the canonical double-helix. Here we detail a method to investigate how polymerase pausing in the Pacific Biosciences sequencer reads can be related to DNA structure. The Pacific Biosciences sequencer uses optics to view a polymerase and its interaction with a single DNA molecule in real-time, offering a uni...