T cells may recognize glycolipids and lipids of bacterial and self origin associated with the CD1 antigen-presenting molecules. Understanding the mechanisms governing CD1-self glycolipid interaction will provide information on the molecular rules of glycolipid presentation and suggest new approaches to immunotherapy.

Quantitative study of the S III-antibody system........................... 52 Precipitation in protein-antiprotein systems............................... 60 Specific bacterial agglutination....................................... 63 Some consequences of the quantitative theory............................. 64 Molecular formulas for antigen-antibody compounds....................... 70 Analysis of ...

Approved: ____________________________________ Thesis Supervisor ____________________________________ Title and Department ____________________________________ Date 1 ORAL MUCOSAL LIPIDS ARE ANTIBACTERIAL AGAINST PORPHYROMONAS GINGIVALIS, INDUCE ULTRASTRUCTURAL DAMAGE, AND ALTER BACTERIAL LIPID AND PROTEIN COMPOSITIONS by Carol Lea Fischer A thesis submitted in partial fulfillment of the requir...

Purpose: To evaluate the application of bacterial liposomes and archaeosomes as a novel drug delivery system for in vitro cytoplasmic delivery of molecules into cancer cells. Methods: Bacterial membrane lipids were extracted using chloroform and methanol. Bacterial liposomes and archaeosomes of E. coli, Acidianus brierleyi and Sulfolobus acidocaldarius were prepared using film method and their ...

Posttranslational modification of proteins by lipidation is a common process in biological systems. Lipids provide protein stability, interaction with other membrane components, and in some cases, due to reversibility of the process, a mechanism for regulating protein localization and function. Bacterial lipoproteins possess fatty acids at their amino-termini that are derived from phospholipids...

To determine the phospholipid requirement of the preprotein translocase in vitro, the Escherichia coli SecYEG complex was purified in a delipidated form using the detergent dodecyl maltoside. SecYEG was reconstituted into liposomes composed of defined synthetic phospholipids, and proteoliposomes were analyzed for their preprotein translocation and SecA translocation ATPase activity. The activit...

Despite the fact that many nuclear receptors are ligand dependent, the existence of obligate regulatory ligands is debated for some receptors, including steroidogenic factor 1 (SF-1). Although fortuitously bound bacterial phospholipids were discovered in the structures of the SF-1 ligand-binding domain (LBD), these lipids might serve merely as structural ligands. Thus, we examined whether exoge...