The variation in bacterial, fungal and total microbial biomass and activity was studied together with the abundance of soil nematodes and microarthropods after the addition of substrates differing in nitrogen availability to a soddy-podzolic soil. The experiments were carried out in microcosms with native and defaunated soil to evaluate stimulatory and suppressive effects of the microfauna on s...

We have developed a reliable high-throughput plasmid isolation system using a 96-well plate format. This system combines a novel glass bead micro-mixing method with modified alkaline lysis and Sephacryl S-500 DNA purification procedures. Mechanical forces generated by vortexing glass beads inside each well of the 96-well plates ensure that the bacterial pellets are homogeneously resuspended, th...

Pathogenic micro-organisms have become the main problem in today’s world. All microbes are getting resistant to antibiotics. This is due to the formation of biofilm layer above the micro-organisms. Learning the characteristics of biofilm can help us in treating the infectious disease induced via micro-organisms. They affect human’s life in some or the other way. For example, the plaque formed i...

The isolation and analysis of single prokaryotic cells down to 1 μm and less in size poses a special challenge and requires micro-engineered devices to handle volumes in the picoliter to nanoliter range. Here, an advanced Single-Cell Printer (SCP) was applied for automated and label-free isolation and deposition of bacterial cells encapsulated in 35 pl droplets by inkjet-like printing. To achie...

to hand, and it appears to be one of growing importance. We therefore refer to the subject again. The idea that vitamins could be synthesized in the gut was suggested about 30 years ago, and the occurrence of it in the gut of animals was first demonstrated 20 years ago. The production of vitamins in the gut of animals has been attributed to the presence of various micro-organisms in the gut. Vi...

Sucrose is an important dietary factor in cariogenic biofilm formation and subsequent initiation of dental caries. This study investigated the functional relationships between sucrose concentration and Streptococcus mutans adherence and biofilm formation. Changes in morphological characteristics of the biofilms with increasing sucrose concentration were also evaluated. S. mutans biofilms were f...

On agar surface, bacterial daughter cells form a 4-cell array after the first two rounds of division, and this phenomenon has been previously attributed to a balancing of interactions among the daughter bacteria and the underneath agar. We studied further the organization and development of colony after additional generations. By confocal laser scanning microscopy and real-time imaging, we obse...

background and objectives: bacterial resistance to antibiotics has motivated the researchers to evaluate the novel anti-bac- terial compounds such as some thiazole and imidazole derivatives. thereby, in this work, we investigated the anti-bacterial effects of one new thiazole and two new imidazole derivatives on bacillus cereus , listeria monocytogenes , escherichia coli, salmonella typhimurium...

background and objectives:infection of pleural fluid is a common disease and because of antibiotic administration, the microbiology of this fluid has changed. the aim of this study was to determine the common bacteria and suitable antibiotics for treatment in pleural effusion (pe). materials and methods: in this cross sectional study, 1210 samples with exudative features were cultured for poss...