Some chemicals used by human such as food additives are mutagenic and mutagenicity effects analysis of them is very important because of considerable consumption by man. One of the most common methods used in the recognition of the chemicals' mutagenicity is Salmonella Typhimurium Reverse Mutation (Ames) test. The purpose of the present study is mutagenicity effects analysis of sodium nitrite, ...

The aim of this study was to use molecular identification methods, such as 16S RNA gene sequence and reverse-capture checkerboard hybridization, for identification of the bacteria associated with dental caries and with dental health in a subset of 204 twins aged 1.5 to 7 years old. A total of 448 plaque samples (118 collected from caries-free subjects and 330 from caries-active subjects) were u...

background: small intestinal bacterial overgrowth (sibo) may have a role in the pathophysiology of irritable bowel syndrome (ibs). so, the aim of this study was to assess the association between sibo and ibs by using glucose breath test (gbt) in kerman city as the first study in iranian population. method: 107 patients with ibs and 107 healthy individualswere enrolled in our study. all the part...

Cystic fibrosis is an autosomal recessive disease caused by a wide spectrum of mutations in the gene encoding for the cystic fibrosis transmembrane conductance regulator protein. These mutations that correlate with different phenotypes, vary in their frequency and distribution in different populations. In this study missense mutation R117H that associated with the different clinical symptoms wa...

the objective of the present study was to determine the grades of nonspecific bacterial infection of genitalia of repeat breeding cattle by a simple and rapid test under field condition. for this purpose, a total of 100 crossbred jersey cows comprising of 80 repeat breeding animals presented for treatment and 20 normal cyclic (control group) animals presented for artificial insemination at thei...

The mutagenic effect of multi-walled carbon nanotubes (MWCNTs) characterised by small surface/volume ratio, high diameter and less than 0.1% of metal contaminants was evaluated by the bacterial reverse mutation assay (Ames test) on Salmonella typhimurium TA 98 and TA 100 strains, and on Escherichia coli WP2uvrA strain, in presence and in absence of the metabolic activation system S9. A prelimin...

Background: Cystic fibrosis (CF) is a common autosomal recessive disorder that affects many body systems and is produced by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. CF is also the most frequently inherited disorder in the West. The aim of this study was to detect the mutations in the CFTR gene in two Iranian families with CF. Methods: After DNA extractio...

background and aims: lamivudine is amongst the antiviral for drug chronic hepatitis b treatment. during therapy with lamivudine, variants may emerge with ymdd mutation in the reverse transcriptase (rt) region of polymerase gene. this mutation might have a role in drug resistant for hbv. materials and methods: hbv dna extraction from serum sample of 88 patients, were subjected to nested pcr for ...

I n the past two decades, intense efforts have been devoted to uncovering the mechanisms responsible for the maintenance of telomeres in eukaryotic cells. These efforts have led to the identification of an unusual reverse transcriptase (RT), named telomerase, that uses an integral RNA subunit as template to synthesize a short reiterated sequence at the ends of eukaryotic chromosomes (Fig. 1A) (...